Product nameAnti-USP3 antibody
See all USP3 primary antibodies
DescriptionRabbit polyclonal to USP3
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chicken, Dog, Pig, Macaque monkey
Synthetic peptide corresponding to Human USP3 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following cell lysates: U20S; HeLa Nuclear; Jurkat.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500. Use with paraformaldehyde-fixed cells, permeabilized with 0.5% Triton X100-PBS.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
FunctionHydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Tissue specificityExpressed in all tissues examined, with strongest expression in pancreas.
Sequence similaritiesBelongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger.
DomainBoth protease activity and an intact zinc finger are required for H2A monodeubiquitination.
Cellular localizationNucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
- Information by UniProt
- Deubiquitinating enzyme 3 antibody
- EC 188.8.131.52 antibody
- SIH003 antibody
All lanes : Anti-USP3 antibody (ab101473) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab101473 (1/500) staining USP3 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
This product has been referenced in:
- Zhou Q et al. Ubiquitin-specific protease 3 targets TRAF6 for deubiquitination and suppresses IL-1ß induced chondrocyte apoptosis. Biochem Biophys Res Commun 514:482-489 (2019). Read more (PubMed: 31056254) »
- Wang Z et al. Downregulated USP3 mRNA functions as a competitive endogenous RNA of SMAD4 by sponging miR-224 and promotes metastasis in colorectal cancer. Sci Rep 7:4281 (2017). WB ; Human . Read more (PubMed: 28655924) »