Anti-USP3 antibody (ab229348)
Key features and details
- Rabbit polyclonal to USP3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP3 antibody
See all USP3 primary antibodies -
Description
Rabbit polyclonal to USP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
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Immunogen
Recombinant fragment within Human USP3 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y6I4 -
Positive control
- ICC/IF: K562 cells. WB: HEK-293T, A431 and HeLa whole cell lysate; USP3-transfected HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab229348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/20000. Predicted molecular weight: 59 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. -
Tissue specificity
Expressed in all tissues examined, with strongest expression in pancreas. -
Sequence similarities
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger. -
Domain
Both protease activity and an intact zinc finger are required for H2A monodeubiquitination. -
Cellular localization
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin. - Information by UniProt
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Database links
- Entrez Gene: 9960 Human
- Entrez Gene: 235441 Mouse
- Entrez Gene: 363084 Rat
- GenBank: NM_006537 Human
- GenBank: NM_144937 Mouse
- Omim: 604728 Human
- SwissProt: Q9Y6I4 Human
- SwissProt: Q91W36 Mouse
see all -
Alternative names
- Deubiquitinating enzyme 3 antibody
- EC 3.1.2.15 antibody
- SIH003 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-USP3 antibody (ab229348)
K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for USP3 (green) using ab229348 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Western blot - Anti-USP3 antibody (ab229348)All lanes : Anti-USP3 antibody (ab229348) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
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Western blot - Anti-USP3 antibody (ab229348)All lanes : Anti-USP3 antibody (ab229348) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Human USP3-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229348 has not yet been referenced specifically in any publications.
