Anti-USP3 antibody (ab229348)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-USP3 antibody
See all USP3 primary antibodies -
Description
Rabbit polyclonal to USP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
-
Immunogen
Recombinant fragment within Human USP3 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y6I4 -
Positive control
- ICC/IF: K562 cells. WB: HEK-293T, A431 and HeLa whole cell lysate; USP3-transfected HEK-293T whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab229348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/20000. Predicted molecular weight: 59 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. -
Tissue specificity
Expressed in all tissues examined, with strongest expression in pancreas. -
Sequence similarities
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger. -
Domain
Both protease activity and an intact zinc finger are required for H2A monodeubiquitination. -
Cellular localization
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin. - Information by UniProt
-
Database links
- Entrez Gene: 9960 Human
- Entrez Gene: 235441 Mouse
- Entrez Gene: 363084 Rat
- GenBank: NM_006537 Human
- GenBank: NM_144937 Mouse
- Omim: 604728 Human
- SwissProt: Q9Y6I4 Human
- SwissProt: Q91W36 Mouse
see all -
Alternative names
- Deubiquitinating enzyme 3 antibody
- EC 3.1.2.15 antibody
- SIH003 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-USP3 antibody (ab229348)K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for USP3 (green) using ab229348 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
-
Western blot - Anti-USP3 antibody (ab229348)All lanes : Anti-USP3 antibody (ab229348) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
-
Western blot - Anti-USP3 antibody (ab229348)All lanes : Anti-USP3 antibody (ab229348) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Human USP3-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
References
ab229348 has not yet been referenced specifically in any publications.
