Key features and details
- Rabbit polyclonal to USP3
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP3 antibody
See all USP3 primary antibodies
DescriptionRabbit polyclonal to USP3
Tested applicationsSuitable for: IHC-P, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide (Human) mapped to a region between residues 471 and 521
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.5% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use a concentration of 2 - 5 µg/ml.|
FunctionHydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Tissue specificityExpressed in all tissues examined, with strongest expression in pancreas.
Sequence similaritiesBelongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger.
DomainBoth protease activity and an intact zinc finger are required for H2A monodeubiquitination.
Cellular localizationNucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
- Information by UniProt
- Deubiquitinating enzyme 3 antibody
- EC 22.214.171.124 antibody
- SIH003 antibody
ab80597 has not yet been referenced specifically in any publications.