Overview

  • Product name

  • Description

    Rabbit polyclonal to USP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 471 and 521 of Human USP3 (AAD42992.1).

  • Positive control

    • Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Metastatic Lymph Node, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma and Stomach Adenocarcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
    • Tissue specificity

      Expressed in all tissues examined, with strongest expression in pancreas.
    • Sequence similarities

      Belongs to the peptidase C19 family. USP3 subfamily.
      Contains 1 UBP-type zinc finger.
    • Domain

      Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.
    • Cellular localization

      Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
    • Information by UniProt
    • Database links

    • Alternative names

      • Deubiquitinating enzyme 3 antibody
      • EC 3.1.2.15 antibody
      • SIH003 antibody
      • Ubiquitin carboxyl-terminal hydrolase 3 antibody
      • Ubiquitin specific peptidase 3 antibody
      • Ubiquitin specific processing protease 3 antibody
      • Ubiquitin Specific Protease 3 antibody
      • Ubiquitin thioesterase 3 antibody
      • Ubiquitin thiolesterase 3 antibody
      • Ubiquitin-specific-processing protease 3 antibody
      • UBP antibody
      • UBP3_HUMAN antibody
      • Usp3 antibody
      see all

    Images

    • ab84702, at 1/100 dilution, staining USP3 in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry using a Red fluorescent goat anti-rabbit IgG highly cross adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
    • ab84702, at 1/500 dilution, staining USP3 in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry using DAB staining.
    • ab84702, at 1/100 dilution, staining USP3 in formalin-fixed, paraffin-embedded human pancreatic islet cell tumor by Immunohistochemistry using DAB staining.

    References

    ab84702 has not yet been referenced specifically in any publications.

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