Anti-USP33 antibody (ab237510)
Key features and details
- Rabbit polyclonal to USP33
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP33 antibody
See all USP33 primary antibodies -
Description
Rabbit polyclonal to USP33 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human USP33 aa 251-550.
Database link: Q8TEY7 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Human prostate cancer tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Predicted molecular weight: 107 kDa.
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Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 107 kDa. |
Target
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Function
Deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the peptidase C19 family. USP20/USP33 subfamily.
Contains 2 DUSP domains.
Contains 1 UBP-type zinc finger. -
Domain
The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue. -
Post-translational
modificationsUbiquitinated via a VHL-dependent pathway for proteasomal degradation. -
Cellular localization
Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear. - Information by UniProt
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Database links
- Entrez Gene: 23032 Human
- SwissProt: Q8TEY7 Human
- Unigene: 726151 Human
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Alternative names
- Deubiquitinating enzyme 33 antibody
- EC 3 1 2 15 antibody
- hVDU1 antibody
see all
Images
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Anti-USP33 antibody (ab237510) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 107 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for USP33 (green) at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
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Paraffin-embedded human prostate cancer tissue stained for USP33 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab237510 has been referenced in 1 publication.
- Li Q et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun 13:7677 (2022). PubMed: 36509766