• Product name

  • Description

    Rabbit polyclonal to USP33
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human USP33 (C terminal). A region between residue 900 and the C-terminus (residue 942) of human Ubiquitin Specific Peptidase 33 (NP_055832.3).
    Database link: 23032



Our Abpromise guarantee covers the use of ab71716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 107 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.


  • Function

    Deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP20/USP33 subfamily.
    Contains 2 DUSP domains.
    Contains 1 UBP-type zinc finger.
  • Domain

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue.
  • Post-translational

    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
  • Cellular localization

    Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 33 antibody
    • EC 3 1 2 15 antibody
    • hVDU1 antibody
    • KIAA1097 antibody
    • MGC16868 antibody
    • OTTHUMP00000011234 antibody
    • OTTHUMP00000011330 antibody
    • OTTHUMP00000011331 antibody
    • pVHL interacting deubiquitinating enzyme 1 antibody
    • Ubiquitin carboxyl terminal hydrolase 33 antibody
    • Ubiquitin carboxyl-terminal hydrolase 33 antibody
    • Ubiquitin specific peptidase 33 antibody
    • Ubiquitin specific processing protease 33 antibody
    • ubiquitin specific protease 33 antibody
    • Ubiquitin thioesterase 33 antibody
    • Ubiquitin thiolesterase 33 antibody
    • Ubiquitin-specific-processing protease 33 antibody
    • UBP33_HUMAN antibody
    • USP 33 antibody
    • Usp33 antibody
    • VDU 1 antibody
    • VDU1 antibody
    • VHL interacting deubiquitinating enzyme 1 antibody
    • VHL-interacting deubiquitinating enzyme 1 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP33 with ab71716 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-USP33 antibody (ab71716) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 107 kDa
    Observed band size: 117 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 63 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes


ab71716 has not yet been referenced specifically in any publications.

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