Product nameAnti-USP35 antibody
See all USP35 primary antibodies
DescriptionRabbit polyclonal to USP35
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between residues 967 and 1017 of human USP35 (CAE51937.1).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86791 was affinity purified using an epitope specific to USP35 immobilized on solid support.
Our Abpromise guarantee covers the use of ab86791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 113 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityExpressed in testis, pancreas and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C19 family.
- Information by UniProt
- Deubiquitinating enzyme 35 antibody
- Gm1088 antibody
- Gm493 antibody
All lanes : Anti-USP35 antibody (ab86791) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 3 minutes
Detection of USP35 by Western Blot of Immunprecipitate.
ab86791 at 1 µg/ml staining USP35 in HeLa whole cell lysate immunoprecipitated using ab86791 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Liu C et al. USP35 activated by miR let-7a inhibits cell proliferation and NF-?B activation through stabilization of ABIN-2. Oncotarget 6:27891-906 (2015). Read more (PubMed: 26348204) »
- Zhang Y et al. Effects of 1.8 GHz radiofrequency radiation on protein expression in human lens epithelial cells. Hum Exp Toxicol N/A:N/A (2013). WB ; Human . Read more (PubMed: 23338683) »