Overview

  • Product name

  • Description

    Rabbit polyclonal to USP36
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human USP36 (C terminal). The exact sequence is proprietary.
    Database link: Q9P275

  • Positive control

    • WB: U-2 OS, mouse MEF and INS-1 cell lysates. Flow Cyt: U-2 OS cells. ICC/IF: U-2 OS cells transfected with Flag-USP36.

Properties

Applications

Our Abpromise guarantee covers the use of ab236452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 123 kDa.
Flow Cyt 1/200.
ICC/IF 1/2000.

Target

  • Function

    May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).
  • Tissue specificity

    Broadly expressed.
  • Sequence similarities

    Belongs to the peptidase C19 family.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 1 antibody
    • Deubiquitinating enzyme 36 antibody
    • DUB1 antibody
    • FLJ12851 antibody
    • KIAA1453 antibody
    • Ubiquitin carboxyl-terminal hydrolase 36 antibody
    • Ubiquitin specific peptidase 36 antibody
    • Ubiquitin specific processing protease 36 antibody
    • Ubiquitin thioesterase 36 antibody
    • Ubiquitin thiolesterase 36 antibody
    • Ubiquitin-specific-processing protease 36 antibody
    • UBP36_HUMAN antibody
    • Usp36 antibody
    see all

Images

  • All lanes : Anti-USP36 antibody (ab236452) at 1/2000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA
    Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with USP36 siRNA
    Lane 3 : Mouse MEF cell lysate
    Lane 4 : Rat INS-1 cell lysate

    Predicted band size: 123 kDa

  • 2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with Flag-USP36 stained for USP36 (red) using ab236452 at 1/2000 dilution in ICC/IF. Flag (green). Right panel shows the merged images with DAPI.

  • Flow cytometric analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cell line labeling USP36 with ab236452 at 1/200 dilution (green) compared with a negative control with no primary antibody (blue).

References

ab236452 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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