Key features and details
- Rabbit polyclonal to USP36
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-USP36 antibody
DescriptionRabbit polyclonal to USP36
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human USP36 (C terminal). The exact sequence is proprietary.
Database link: Q9P275
- WB: U-2 OS, mouse MEF and INS-1 cell lysates. Flow Cyt: U-2 OS cells. ICC/IF: U-2 OS cells transfected with Flag-USP36.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab236452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 123 kDa.|
FunctionMay be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).
Tissue specificityBroadly expressed.
Sequence similaritiesBelongs to the peptidase C19 family.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Deubiquitinating enzyme 1 antibody
- Deubiquitinating enzyme 36 antibody
- DUB1 antibody
All lanes : Anti-USP36 antibody (ab236452) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with USP36 siRNA
Lane 3 : Mouse MEF cell lysate
Lane 4 : Rat INS-1 cell lysate
Predicted band size: 123 kDa
2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with Flag-USP36 stained for USP36 (red) using ab236452 at 1/2000 dilution in ICC/IF. Flag (green). Right panel shows the merged images with DAPI.
Flow cytometric analysis of U-2 OS (human bone osteosarcoma epithelial cell line) cell line labeling USP36 with ab236452 at 1/200 dilution (green) compared with a negative control with no primary antibody (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236452 has not yet been referenced specifically in any publications.