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  1. Link

    usp37-antibody-ab190184.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination
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Anti-USP37 antibody (ab190184)

  • Datasheet
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Western blot - Anti-USP37 antibody (ab190184)
  • Western blot - Anti-USP37 antibody (ab190184)

Key features and details

  • Rabbit polyclonal to USP37
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Isotype: IgG

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Overview

  • Product name

    Anti-USP37 antibody
    See all USP37 primary antibodies
  • Description

    Rabbit polyclonal to USP37
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to Human USP37 aa 1-300.
    Database link: Q86T82

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • U2OS cells transfected with USP37 siRNA lysate, Mouse embryo fibroblasts cell lysate and INS1 cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000. Predicted molecular weight: 110 kDa.
Notes
WB
1/2000. Predicted molecular weight: 110 kDa.

Target

  • Tissue specificity

    Expressed in brain and prostate.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 3 UIM (ubiquitin-interacting motif) repeats.
  • Target information above from: UniProt accession Q86T82 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57695 Human
    • Entrez Gene: 319651 Mouse
    • Entrez Gene: 316522 Rat
    • SwissProt: Q86T82 Human
    • SwissProt: Q8C0R0 Mouse
    • Unigene: 166068 Human
    • Unigene: 471601 Mouse
    • Unigene: 66568 Mouse
    • Alternative names

      • Deubiquitinating enzyme 3 antibody
      • Deubiquitinating enzyme 37 antibody
      • EC 3.1.2.15 antibody
      • KIAA1594 antibody
      • MGC117261 antibody
      • OTTHUMP00000164146 antibody
      • tmp_locus_50 antibody
      • Ubiquitin carboxyl-terminal hydrolase 37 antibody
      • Ubiquitin specific peptidase 37 antibody
      • Ubiquitin specific processing protease 37 antibody
      • Ubiquitin specific protease 37 antibody
      • Ubiquitin thioesterase 37 antibody
      • Ubiquitin thiolesterase 37 antibody
      • Ubiquitin-specific-processing protease 37 antibody
      • UBP37_HUMAN antibody
      • USP37 antibody
      see all

    Images

    • Western blot - Anti-USP37 antibody (ab190184)
      Western blot - Anti-USP37 antibody (ab190184)
      All lanes : Anti-USP37 antibody (ab190184) at 1/2000 dilution

      Lane 1 : Human U2OS cell lysate si Control
      Lane 2 : Human U2OS cells transfected with USP37 siRNA
      Lane 3 : Mouse Embryo Fibroblaste cell Lysate
      Lane 4 : Rat INS1 cell lysate

      Lysates/proteins at 40 µg per lane.

      Predicted band size: 110 kDa

    • Western blot - Anti-USP37 antibody (ab190184)
      Western blot - Anti-USP37 antibody (ab190184)
      All lanes : Anti-USP37 antibody (ab190184) at 1/2000 dilution

      Lane 1 : Xenopus laevis crude egg extracts


      Lysates/proteins at 50 µg per lane.

      Predicted band size: 110 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (4)

    Publishing research using ab190184? Please let us know so that we can cite the reference in this datasheet.

    ab190184 has been referenced in 4 publications.

    • Li W  et al. Intermittent hypoxia-induced downregulation of microRNA-320b promotes lung cancer tumorigenesis by increasing CDT1 via USP37. Mol Ther Nucleic Acids 24:528-541 (2021). IHC-P ; Human . PubMed: 33898105
    • Hong K  et al. USP37 promotes deubiquitination of HIF2a in kidney cancer. Proc Natl Acad Sci U S A N/A:N/A (2020). WB ; Human . PubMed: 32461361
    • Xiao Z  et al. USP37 is a SNAI1 deubiquitinase. Am J Cancer Res 9:2749-2759 (2019). WB ; Human . PubMed: 31911859
    • Hernández-Pérez S  et al. USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication. Mol Oncol N/A:N/A (2016). WB ; Human . PubMed: 27296872

    Customer reviews and Q&As

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