All lanes : Anti-USP38 antibody (ab72244) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 117 kDa Observed band size: 117 kDa Additional bands at: 31 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of USP38.
Lane 1: ab72244 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72244 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.
Liu Q et al. Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses. Sci Adv4:eaar2824 (2018).
Read more (PubMed: 29732405) »