Key features and details
- Rabbit polyclonal to USP38
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP38 antibody
DescriptionRabbit polyclonal to USP38
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Pig, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residues 700 and 750 of human USP38 (NP_115946.2)
- Whole cell lysate from Hela and 293T cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2,000 - 1/10,000. Detects bands of approximately 31, 68, and 117 kDa (predicted molecular weight: 117 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityHighly expressed in skeletal muscle. Expressed in adrenal gland.
Sequence similaritiesBelongs to the peptidase C19 family.
- Information by UniProt
- KIAA1891 antibody
- Deubiquitinating enzyme 38 antibody
- HP43.8KD antibody
All lanes : Anti-USP38 antibody (ab72244) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 117 kDa
Observed band size: 117 kDa
Additional bands at: 31 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of USP38.
Lane 1: ab72244 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72244 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.
ab72244 has been referenced in 1 publication.
- Liu Q et al. Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses. Sci Adv 4:eaar2824 (2018). PubMed: 29732405