Product nameAnti-USP38 antibody
DescriptionRabbit polyclonal to USP38
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Pig, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residues 700 and 750 of human USP38 (NP_115946.2)
- Whole cell lysate from Hela and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2,000 - 1/10,000. Detects bands of approximately 31, 68, and 117 kDa (predicted molecular weight: 117 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityHighly expressed in skeletal muscle. Expressed in adrenal gland.
Sequence similaritiesBelongs to the peptidase C19 family.
- Information by UniProt
- KIAA1891 antibody
- Deubiquitinating enzyme 38 antibody
- HP43.8KD antibody
All lanes : Anti-USP38 antibody (ab72244) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 117 kDa
Observed band size: 117 kDa
Additional bands at: 31 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of USP38.
Lane 1: ab72244 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72244 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.