Product nameAnti-USP39 antibody [EPR8683]
See all USP39 primary antibodies
DescriptionRabbit monoclonal [EPR8683] to USP39
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human USP39 aa 250-350. The exact sequence is proprietary.
- HeLa, 293T, K562 and Jurkat cell lysates; Human colonic adenocarcinoma tissue; Hela cells
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.37 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 65 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionMay play a role in mRNA splicing. It is unsure if the protein really exhibits hydrolase activity. Could be a competitor of ubiquitin C-terminal hydrolases (UCHs).
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger.
- Information by UniProt
- 65K antibody
- CGI 21 antibody
- CGI-21 antibody
All lanes : Anti-USP39 antibody [EPR8683] (ab131244) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa
Immunofluorescence analysis of HeLa cells labelling USP39 with ab131244 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labelling USP39 with ab131244 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Wu J et al. USP39 regulates DNA damage response and chemo-radiation resistance by deubiquitinating and stabilizing CHK2. Cancer Lett 449:114-124 (2019). Read more (PubMed: 30771428) »
- Wang L et al. USP39 promotes ovarian cancer malignant phenotypes and carboplatin chemoresistance. Int J Oncol 55:277-288 (2019). Read more (PubMed: 31180526) »