Anti-USP39 antibody - N-terminal (ab236453)
Key features and details
- Rabbit polyclonal to USP39 - N-terminal
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP39 antibody - N-terminal
See all USP39 primary antibodies -
Description
Rabbit polyclonal to USP39 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human USP39 (N terminal). The exact sequence is proprietary.
Database link: Q53GS9 -
Positive control
- WB: U-2 OS siCtrl lysate. IP: U-2 OS cell lysate. Flow: U-2 OS cells. ICC/IF: Flag -USP39 transfected U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Purification notes
Crude serum containing sodium azide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 65 kDa.
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ICC/IF |
1/2000.
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Flow Cyt |
1/200.
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IP |
Use at an assay dependent concentration.
Use 3 µl to ~400 µg of extact |
Notes |
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WB
1/2000. Predicted molecular weight: 65 kDa. |
ICC/IF
1/2000. |
Flow Cyt
1/200. |
IP
Use at an assay dependent concentration. Use 3 µl to ~400 µg of extact |
Target
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Function
May play a role in mRNA splicing. It is unsure if the protein really exhibits hydrolase activity. Could be a competitor of ubiquitin C-terminal hydrolases (UCHs). -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10713 Human
- Omim: 611594 Human
- SwissProt: Q53GS9 Human
- Unigene: 654745 Human
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Alternative names
- 65K antibody
- CGI 21 antibody
- CGI-21 antibody
see all
Images
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All lanes : Anti-USP39 antibody - N-terminal (ab236453) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) siUSP39
Lysates/proteins at 40 µg per lane.
Predicted band size: 65 kDaBlock: TBS-T 5% milk. Incubated overnight with primary antibody.
Treated with control or USP39 siRNA to show specificity.
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Top panel: Flag -USP39 transfected U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for USP39 using ab236453 at a dilution of 1/2000 in ICC/IF.
Middle panel: Anti-Flag.
Bottom panel: Merged (+ DAPI) image.
Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.
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Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling USP39 with ab236453 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).
Cells were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.
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USP39 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236453.
Lane 1: U-2 OS whole cell lysate (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236453 in U-2 OS whole cell lysate.
For western blotting ab236453 was used at a 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236453 has not yet been referenced specifically in any publications.