Product nameAnti-USP39 antibody - N-terminal
See all USP39 primary antibodies
DescriptionRabbit polyclonal to USP39 - N-terminal
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human USP39 (N terminal). The exact sequence is proprietary.
Database link: Q53GS9
- WB: U-2 OS siCtrl lysate. IP: U-2 OS cell lysate. Flow: U-2 OS cells. ICC/IF: Flag -USP39 transfected U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification notesCrude serum containing sodium azide.
Our Abpromise guarantee covers the use of ab236453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 65 kDa.|
|IP||Use at an assay dependent concentration.
Use 3 µl to ~400 µg of extact
FunctionMay play a role in mRNA splicing. It is unsure if the protein really exhibits hydrolase activity. Could be a competitor of ubiquitin C-terminal hydrolases (UCHs).
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger.
- Information by UniProt
- 65K antibody
- CGI 21 antibody
- CGI-21 antibody
All lanes : Anti-USP39 antibody - N-terminal (ab236453) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) siUSP39
Lysates/proteins at 40 µg per lane.
Predicted band size: 65 kDa
Block: TBS-T 5% milk. Incubated overnight with primary antibody.
Treated with control or USP39 siRNA to show specificity.
Top panel: Flag -USP39 transfected U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for USP39 using ab236453 at a dilution of 1/2000 in ICC/IF.
Middle panel: Anti-Flag.
Bottom panel: Merged (+ DAPI) image.
Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.
Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling USP39 with ab236453 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).
Cells were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.
USP39 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236453.
Lane 1: U-2 OS whole cell lysate (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236453 in U-2 OS whole cell lysate.
For western blotting ab236453 was used at a 1/2000 dilution.
ab236453 has not yet been referenced specifically in any publications.