Key features and details
- Rabbit polyclonal to USP39 - N-terminal
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-USP39 antibody - N-terminal
See all USP39 primary antibodies
DescriptionRabbit polyclonal to USP39 - N-terminal
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human USP39 (N terminal). The exact sequence is proprietary.
Database link: Q53GS9
- WB: U-2 OS siCtrl lysate. IP: U-2 OS cell lysate. Flow: U-2 OS cells. ICC/IF: Flag -USP39 transfected U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Purification notesCrude serum containing sodium azide.
Our Abpromise guarantee covers the use of ab236453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 65 kDa.|
|IP||Use at an assay dependent concentration.
Use 3 µl to ~400 µg of extact
FunctionMay play a role in mRNA splicing. It is unsure if the protein really exhibits hydrolase activity. Could be a competitor of ubiquitin C-terminal hydrolases (UCHs).
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger.
- Information by UniProt
- 65K antibody
- CGI 21 antibody
- CGI-21 antibody
All lanes : Anti-USP39 antibody - N-terminal (ab236453) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) siUSP39
Lysates/proteins at 40 µg per lane.
Predicted band size: 65 kDa
Block: TBS-T 5% milk. Incubated overnight with primary antibody.
Treated with control or USP39 siRNA to show specificity.
Top panel: Flag -USP39 transfected U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for USP39 using ab236453 at a dilution of 1/2000 in ICC/IF.
Middle panel: Anti-Flag.
Bottom panel: Merged (+ DAPI) image.
Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.
Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling USP39 with ab236453 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).
Cells were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.
USP39 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236453.
Lane 1: U-2 OS whole cell lysate (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236453 in U-2 OS whole cell lysate.
For western blotting ab236453 was used at a 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236453 has not yet been referenced specifically in any publications.