Anti-USP4 antibody (ab245654)

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Overview

  • Product name

    Anti-USP4 antibody
    See all USP4 primary antibodies

  • Description

    Rabbit polyclonal to USP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Monkey, Orangutan, Giant Panda

  • Immunogen

    Synthetic peptide within Human USP4 aa 925-963. The exact sequence is proprietary. AAB72237.1
    Database link: Q13107

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 109 kDa.

Target

  • Function

    Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.

  • Tissue specificity

    Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.

  • Sequence similarities

    Belongs to the peptidase C19 family. USP4 subfamily.
    Contains 1 DUSP domain.

  • Post-translational
    modifications

    Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.

  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
  • Information by UniProt

  • Database links

    • Alternative names

      • Deubiquitinating enzyme 4 antibody
      • MGC149848 antibody
      • MGC149849 antibody
      • Proto oncogene antibody
      • Protooncogene antibody
      • Ubiquitin carboxyl terminal hydrolase 4 antibody
      • Ubiquitin carboxyl-terminal hydrolase 4 antibody
      • Ubiquitin specific peptidase 4 (proto oncogene) antibody
      • Ubiquitin specific peptidase 4 (protooncogene) antibody
      • Ubiquitin specific peptidase 4 antibody
      • Ubiquitin specific processing protease 4 antibody
      • Ubiquitin specific protease 4 (proto oncogene) antibody
      • Ubiquitin specific protease 4 (protooncogene) antibody
      • Ubiquitin specific protease 4 antibody
      • Ubiquitin specific protease proto oncogene antibody
      • Ubiquitin specific protease protooncogene antibody
      • Ubiquitin thioesterase 4 antibody
      • Ubiquitin thiolesterase 4 antibody
      • Ubiquitin-specific-processing protease 4 antibody
      • Ubiquitous nuclear protein homolog antibody
      • UBP4_HUMAN antibody
      • UNP antibody
      • UNPH antibody
      • USP 4 antibody
      • USP4 antibody
      see all

    Images

    • Western blot - Anti-USP4 antibody (ab245654)
      Western blot - Anti-USP4 antibody (ab245654)
      All lanes : Anti-USP4 antibody (ab245654) at 0.4 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 109 kDa


      Exposure time: 30 seconds


      Prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-USP4 antibody (ab245654)
      Immunoprecipitation - Anti-USP4 antibody (ab245654)

      USP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

      ab245654 used for IP at 6 µg per reaction. For WB 1 µg/ml.

      Lane 1: ab245654 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Chemiluminescence detection: 30 seconds.

    References

    ab245654 has not yet been referenced specifically in any publications.

    Publishing research using ab245654? Please let us know so that we can cite the reference in this datasheet.

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