Overview

  • Product name

    Anti-USP4 antibody [EPR13846]
    See all USP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13846] to USP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human USP4 aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13107

  • Positive control

    • HuT-78 and THP1 cell lysates,
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 109 kDa.
ICC/IF 1/250 - 1/500.

Target

  • Function

    Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.
  • Tissue specificity

    Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP4 subfamily.
    Contains 1 DUSP domain.
  • Post-translational
    modifications

    Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 4 antibody
    • MGC149848 antibody
    • MGC149849 antibody
    • Proto oncogene antibody
    • Protooncogene antibody
    • Ubiquitin carboxyl terminal hydrolase 4 antibody
    • Ubiquitin carboxyl-terminal hydrolase 4 antibody
    • Ubiquitin specific peptidase 4 (proto oncogene) antibody
    • Ubiquitin specific peptidase 4 (protooncogene) antibody
    • Ubiquitin specific peptidase 4 antibody
    • Ubiquitin specific processing protease 4 antibody
    • Ubiquitin specific protease 4 (proto oncogene) antibody
    • Ubiquitin specific protease 4 (protooncogene) antibody
    • Ubiquitin specific protease 4 antibody
    • Ubiquitin specific protease proto oncogene antibody
    • Ubiquitin specific protease protooncogene antibody
    • Ubiquitin thioesterase 4 antibody
    • Ubiquitin thiolesterase 4 antibody
    • Ubiquitin-specific-processing protease 4 antibody
    • Ubiquitous nuclear protein homolog antibody
    • UBP4_HUMAN antibody
    • UNP antibody
    • UNPH antibody
    • USP 4 antibody
    • USP4 antibody
    see all

Images

  • Immunofluorescent staining of -20℃ Acetone-fixed THP1 cells labeling USP4 using ab181105 at a 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/200 dilution and counter stained with DAPI.

  • All lanes : Anti-USP4 antibody [EPR13846] (ab181105) at 1/1000 dilution

    Lane 1 : HuT-78 cell lysate
    Lane 2 : THP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

References

This product has been referenced in:

See 1 Publication for this product

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