Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13846] to USP4
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-USP4 antibody [EPR13846]
See all USP4 primary antibodies
DescriptionRabbit monoclonal [EPR13846] to USP4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human USP4 aa 900 to the C-terminus. The exact sequence is proprietary.
Database link: Q13107
- HuT-78 and THP1 cell lysates,
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 109 kDa.|
|ICC/IF||1/250 - 1/500.|
FunctionHydrolase that deubiquitinates target proteins such as the receptor ADORA2A and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.
Tissue specificityOverexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.
Sequence similaritiesBelongs to the peptidase C19 family. USP4 subfamily.
Contains 1 DUSP domain.
modificationsMonoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.
- Information by UniProt
- Deubiquitinating enzyme 4 antibody
- MGC149848 antibody
- MGC149849 antibody
Immunofluorescent staining of -20℃ Acetone-fixed THP1 cells labeling USP4 using ab181105 at a 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/200 dilution and counter stained with DAPI.
All lanes : Anti-USP4 antibody [EPR13846] (ab181105) at 1/1000 dilution
Lane 1 : HuT-78 cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
ab181105 has been referenced in 1 publication.
- Guo W et al. Up-regulated deubiquitinase USP4 plays an oncogenic role in melanoma. J Cell Mol Med 22:2944-2954 (2018). PubMed: 29542252