Overview

  • Product name

    Anti-USP40 antibody
  • Description

    Rabbit polyclonal to USP40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 755-900 of Human USP40.

  • Positive control

    • Human tonsil tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab121234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use Buffer at pH 6 for antigen retrieval.

Target

  • Function

    May be catalytically inactive.
  • Tissue specificity

    Broadly expressed.
  • Sequence similarities

    Belongs to the peptidase C19 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 40 antibody
    • Ubiquitin carboxyl-terminal hydrolase 40 antibody
    • Ubiquitin thiolesterase 40 antibody
    • Ubiquitin-specific-processing protease 40 antibody
    • UBP40_HUMAN antibody
    • Usp40 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles and focal adhesions. Recommended concentration of ab121234 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121234, at 1/75 dilution, staining USP40 in Human tonsil tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Note: shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.

References

ab121234 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Colon and Prostate)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
60 µg
Specification
Colon and Prostate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Keren Tazat

Verified customer

Submitted Feb 27 2018

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - other (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 06 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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