Anti-USP42 antibody (ab121254)
Key features and details
- Rabbit polyclonal to USP42
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP42 antibody -
Description
Rabbit polyclonal to USP42 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to amino acids 454-580 of Human USP42 (Q9H9J4).
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Positive control
- Human kidney tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
Target
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Function
Deubiquitinating enzyme which may play an important role during spermatogenesis. -
Tissue specificity
Broadly expressed. -
Sequence similarities
Belongs to the peptidase C19 family. - Information by UniProt
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Database links
- Entrez Gene: 84132 Human
- SwissProt: Q9H9J4 Human
- Unigene: 31856 Human
- Unigene: 707731 Human
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Alternative names
- Deubiquitinating enzyme 42 antibody
- FLJ12697 antibody
- Ubiquitin carboxyl-terminal hydrolase 42 antibody
see all
Images
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Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121254 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical staining of parffin embedded Human kidney, using 1/60 ab121254, shows strong nuclear cytoplasmic positivity in cells in tubules and cells in glomeruli.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121254 has not yet been referenced specifically in any publications.