Overview

  • Product name

  • Description

    Mouse polyclonal to USP46
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human USP46 protein (NP_073743.2).

  • Positive control

    • Human kidney tissue lysate. USP46 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • Function

    Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
  • Tissue specificity

    Broadly expressed.
  • Sequence similarities

    Belongs to the peptidase C19 family. USP12/USP46 subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 46 antibody
    • FLJ11850 antibody
    • FLJ12552 antibody
    • FLJ14283 antibody
    • FLJ39393 antibody
    • Ubiquitin carboxyl terminal hydrolase 46 antibody
    • Ubiquitin carboxyl-terminal hydrolase 46 antibody
    • Ubiquitin specific peptidase 46 antibody
    • Ubiquitin specific processing protease 46 antibody
    • Ubiquitin specific protease 46 antibody
    • Ubiquitin thioesterase 46 antibody
    • Ubiquitin thiolesterase 46 antibody
    • Ubiquitin-specific-processing protease 46 antibody
    • UBP46_HUMAN antibody
    • USP46 antibody
    see all

Images

References

This product has been referenced in:

  • Zhang Y  et al. Amyloid-ß Induces AMPA Receptor Ubiquitination and Degradation in Primary Neurons and Human Brains of Alzheimer's Disease. J Alzheimers Dis 62:1789-1801 (2018). Read more (PubMed: 29614651) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (MCF7)
Specification
MCF7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% triton-x 10min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Nov 08 2010

Application
Western blot
Sample
Human Cell lysate - other (HELA)
Loading amount
30 µg
Specification
HELA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Nov 08 2010

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