Key features and details
- Mouse monoclonal [CL0364] to USP46
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-USP46 antibody [CL0364]
See all USP46 primary antibodies
DescriptionMouse monoclonal [CL0364] to USP46
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
EpitopeBinds to an epitope located within the peptidesequence TNETRCLNCE as determined by overlapping synthetic peptides.
- ICC/IF: U-2 OS cells. WB: U-251 MG cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab244215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.|
FunctionDeubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
Tissue specificityBroadly expressed.
Sequence similaritiesBelongs to the peptidase C19 family. USP12/USP46 subfamily.
- Information by UniProt
- Deubiquitinating enzyme 46 antibody
- FLJ11850 antibody
- FLJ12552 antibody
A-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for USP46 (green) using ab244215 at 4 µg/ml in ICC/IF. Microtubule and nuclear probes are visualized in red and blue respectively.
Anti-USP46 antibody [CL0364] (ab244215) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 42 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244215 has not yet been referenced specifically in any publications.