• Product name
  • Description
    Rabbit polyclonal to USP47
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human USP47.

  • Positive control
    • This antibody gave a positive signal in NIH3T3 whole cell lysates as well as both Human and Mouse Skeletal Muscle tissue lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab109485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 157 kDa).


  • Function
    Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues.
  • Tissue specificity
    Expressed in skeletal muscle, heart and testis.
  • Sequence similarities
    Belongs to the peptidase C19 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 47 antibody
    • TFRP antibody
    • Trf TATA binding protein related factor proximal homolog antibody
    • Trf TATA binding protein related factor proximal homolog Drosophila antibody
    • Ubiquitin carboxyl-terminal hydrolase 47 antibody
    • Ubiquitin specific peptidase 47 antibody
    • Ubiquitin specific processing protease 47 antibody
    • Ubiquitin specific protease 47 antibody
    • Ubiquitin thioesterase 47 antibody
    • Ubiquitin thiolesterase 47 antibody
    • Ubiquitin-specific-processing protease 47 antibody
    • UBP47_HUMAN antibody
    • USP47 antibody
    see all


  • All lanes : Anti-USP47 antibody (ab109485) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 157 kDa
    Observed band size: 175 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 41 kDa, 57 kDa, 98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes

    The predicted molecular weight of USP47 is 157 kDa (SwissProt), however we expect to observe a banding pattern around 170 kDa. This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109485 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


ab109485 has not yet been referenced specifically in any publications.

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