Anti-USP47 antibody (ab72143)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-USP47 antibody
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DescriptionRabbit polyclonal to USP47
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human USP47 (NP_060414.3)
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Positive control
- HeLa whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab72143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceUSP47 belongs to the peptidase C19 family and may be catalytically inactive although it seems to have kept all necessary active site residues. It is expressed in skeletal muscle, heart and testis and there are 3 named isoforms produced by alternative splicing.
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Database links
- Entrez Gene: 55031 Human
- Entrez Gene: 74996 Mouse
- SwissProt: Q96K76 Human
- SwissProt: Q8BY87 Mouse
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Alternative names
- Deubiquitinating enzyme 47 antibody
- TRF (TATA bidning protein-related factor) proximal homolog antibody
- TRFP antibody
see all
Images
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Western blot - Anti-USP47 antibody (ab72143)All lanes : Anti-USP47 antibody (ab72143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 158 kDa
Observed band size: 170 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 seconds -
Immunoprecipitation - Anti-USP47 antibody (ab72143)Detection of USP47 by Western Blot of Immunprecipitate.
ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
References
This product has been referenced in:
- Sako-Kubota K et al. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell 25:3851-60 (2014). Read more (PubMed: 25253721) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"