Anti-USP47 antibody (ab72143)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-USP47 antibody
See all USP47 primary antibodies -
Description
Rabbit polyclonal to USP47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human USP47 (NP_060414.3)
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Positive control
- HeLa whole cell lysate (ab150035).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab72143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues. -
Tissue specificity
Expressed in skeletal muscle, heart and testis. -
Sequence similarities
Belongs to the peptidase C19 family. - Information by UniProt
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Database links
- Entrez Gene: 55031 Human
- Entrez Gene: 74996 Mouse
- Omim: 614460 Human
- SwissProt: Q96K76 Human
- SwissProt: Q8BY87 Mouse
- Unigene: 577256 Human
- Unigene: 16974 Mouse
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Alternative names
- Deubiquitinating enzyme 47 antibody
- TFRP antibody
- Trf TATA binding protein related factor proximal homolog antibody
see all
Images
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Western blot - Anti-USP47 antibody (ab72143)All lanes : Anti-USP47 antibody (ab72143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 158 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Immunoprecipitation - Anti-USP47 antibody (ab72143)Detection of USP47 by Western Blot of Immunprecipitate.
ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Datasheets and documents
References
This product has been referenced in:
- Yan S et al. LINC00668 promotes tumorigenesis and progression through sponging miR-188-5p and regulating USP47 in colorectal cancer. Eur J Pharmacol 858:172464 (2019). Read more (PubMed: 31233752) »
- Sako-Kubota K et al. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell 25:3851-60 (2014). Read more (PubMed: 25253721) »
