Anti-USP47 antibody (ab72143)
Key features and details
- Rabbit polyclonal to USP47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP47 antibody
See all USP47 primary antibodies -
Description
Rabbit polyclonal to USP47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human USP47 (NP_060414.3)
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Positive control
- HeLa whole cell lysate (ab150035).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab72143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
WB: 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues. -
Tissue specificity
Expressed in skeletal muscle, heart and testis. -
Sequence similarities
Belongs to the peptidase C19 family. - Information by UniProt
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Database links
- Entrez Gene: 55031 Human
- Entrez Gene: 74996 Mouse
- Omim: 614460 Human
- SwissProt: Q96K76 Human
- SwissProt: Q8BY87 Mouse
- Unigene: 577256 Human
- Unigene: 16974 Mouse
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Alternative names
- Deubiquitinating enzyme 47 antibody
- TFRP antibody
- Trf TATA binding protein related factor proximal homolog antibody
see all
Images
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Western blot - Anti-USP47 antibody (ab72143)All lanes : Anti-USP47 antibody (ab72143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 158 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Immunoprecipitation - Anti-USP47 antibody (ab72143)Detection of USP47 by Western Blot of Immunprecipitate.
ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
References (2)
ab72143 has been referenced in 2 publications.
- Yan S et al. LINC00668 promotes tumorigenesis and progression through sponging miR-188-5p and regulating USP47 in colorectal cancer. Eur J Pharmacol 858:172464 (2019). PubMed: 31233752
- Sako-Kubota K et al. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell 25:3851-60 (2014). PubMed: 25253721
