Anti-USP47 antibody (ab84531)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-USP47 antibody
See all USP47 primary antibodies -
Description
Rabbit polyclonal to USP47 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
A region between residue 1237 and the C-terminus (residue 1287) of human USP47 using the numbering given in entry NP_060414.3.
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Positive control
- Human Colon Carcinoma, Linitis Plastica Stomach Cancer, Metastatic Lymph Node, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Small Cell Lung Cancer or Stomach Adenocarcinoma. Mouse Teratoma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified using an epitope specific to USP47 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | ||
ICC/IF |
IHC-P: 1/100 - 1/500.
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues. -
Tissue specificity
Expressed in skeletal muscle, heart and testis. -
Sequence similarities
Belongs to the peptidase C19 family. - Information by UniProt
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Database links
- Entrez Gene: 423032 Chicken
- Entrez Gene: 55031 Human
- Entrez Gene: 74996 Mouse
- Omim: 614460 Human
- SwissProt: E1C1R4 Chicken
- SwissProt: Q96K76 Human
- SwissProt: Q8BY87 Mouse
- Unigene: 577256 Human
see all -
Alternative names
- Deubiquitinating enzyme 47 antibody
- TFRP antibody
- Trf TATA binding protein related factor proximal homolog antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)ab84531 at a 1:500 dilution, staining human USP47 in prostate carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)ab84531 at a 1:500 dilution, staining human USP47 in basal cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)ab84531 at a 1:100 dilution, staining mouse USP47 in teratoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit.
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ab84531 at a 1:100 dilution, staining human USP47 in a FFPE section of prostate adenocarcinoma, using Immunofluorescence. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1:500.
Datasheets and documents
References
ab84531 has not yet been referenced specifically in any publications.