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  1. Link

    usp47-antibody-ab84531.pdf

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Epigenetics and Nuclear Signaling Transcription Mediator Complex
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Anti-USP47 antibody (ab84531)

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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
  • Immunocytochemistry/ Immunofluorescence - Anti-USP47 antibody (ab84531)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-USP47 antibody
    See all USP47 primary antibodies
  • Description

    Rabbit polyclonal to USP47
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    A region between residue 1237 and the C-terminus (residue 1287) of human USP47 using the numbering given in entry NP_060414.3.

  • Positive control

    • Human Colon Carcinoma, Linitis Plastica Stomach Cancer, Metastatic Lymph Node, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Small Cell Lung Cancer or Stomach Adenocarcinoma. Mouse Teratoma.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity purified using an epitope specific to USP47 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Mediator Complex
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human USP47 protein (ab162936)

Applications

Our Abpromise guarantee covers the use of ab84531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notes
    ICC/IF: 1/100.
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.
    Likely to work with frozen sections

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues.
    • Tissue specificity

      Expressed in skeletal muscle, heart and testis.
    • Sequence similarities

      Belongs to the peptidase C19 family.
    • Target information above from: UniProt accession Q96K76 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 423032 Chicken
      • Entrez Gene: 55031 Human
      • Entrez Gene: 74996 Mouse
      • Omim: 614460 Human
      • SwissProt: E1C1R4 Chicken
      • SwissProt: Q96K76 Human
      • SwissProt: Q8BY87 Mouse
      • Unigene: 577256 Human
      • Unigene: 16974 Mouse
      see all
    • Alternative names

      • Deubiquitinating enzyme 47 antibody
      • TFRP antibody
      • Trf TATA binding protein related factor proximal homolog antibody
      • Trf TATA binding protein related factor proximal homolog Drosophila antibody
      • Ubiquitin carboxyl-terminal hydrolase 47 antibody
      • Ubiquitin specific peptidase 47 antibody
      • Ubiquitin specific processing protease 47 antibody
      • Ubiquitin specific protease 47 antibody
      • Ubiquitin thioesterase 47 antibody
      • Ubiquitin thiolesterase 47 antibody
      • Ubiquitin-specific-processing protease 47 antibody
      • UBP47_HUMAN antibody
      • USP47 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
      ab84531 at a 1:500 dilution, staining human USP47 in prostate carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
      ab84531 at a 1:500 dilution, staining human USP47 in basal cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (ab84531)
      ab84531 at a 1:100 dilution, staining mouse USP47 in teratoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using an Immunohistochemistry Accessory Kit.
    • Immunocytochemistry/ Immunofluorescence - Anti-USP47 antibody (ab84531)
      Immunocytochemistry/ Immunofluorescence - Anti-USP47 antibody (ab84531)
      ab84531 at a 1:100 dilution, staining human USP47 in a FFPE section of prostate adenocarcinoma, using Immunofluorescence. Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1:500.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Datasheets and documents

    • Datasheet
  • References

    ab84531 has not yet been referenced specifically in any publications.

    Publishing research using ab84531? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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