Overview

  • Product name

  • Description

    Rabbit polyclonal to USP48
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human USP48 aa 358-508.
    Database link: Q86UV5

  • Positive control

    • WB: HeLa, MCF7, SH-SY5Y, HepG2 and A549 whole cell lysates. IHC-P: Human pancreatic cancer and small intestine tissues. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 119 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 3 DUSP domains.
    Contains 1 ubiquitin-like domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAH67261 antibody
    • Deubiquitinating enzyme 48 antibody
    • DKFZp762M1713 antibody
    • EC 3.1.2.15 antibody
    • Kiaa4202 antibody
    • MGC132556 antibody
    • MGC14879 antibody
    • RAP1GA1 antibody
    • Synaptic ubiquitin specific protease antibody
    • synUSP antibody
    • Ubiquitin carboxyl terminal hydrolase 48 antibody
    • Ubiquitin carboxyl-terminal hydrolase 48 antibody
    • Ubiquitin specific peptidase 48 antibody
    • Ubiquitin specific processing protease 48 antibody
    • Ubiquitin specific protease 31 antibody
    • Ubiquitin specific protease 48 antibody
    • Ubiquitin thioesterase 48 antibody
    • Ubiquitin thiolesterase 48 antibody
    • Ubiquitin-specific-processing protease 48 antibody
    • UBP48_HUMAN antibody
    • USP31 antibody
    • USP48 antibody
    see all

Images

  • All lanes : Anti-USP48 antibody (ab237765) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 119 kDa

  • Paraffin-embedded human small intestine tissue stained for USP48 with ab237765 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human pancreatic cancer tissue stained for USP48 with ab237765 at 1/200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for USP48 (green) using ab237765 at 1/66 dilution in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

References

ab237765 has not yet been referenced specifically in any publications.

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