Product nameAnti-USP48 antibody
See all USP48 primary antibodies
DescriptionRabbit polyclonal to USP48
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residues 985 and 1035 of human USP48 (NP_115612.4).
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72226 was affinity purified using an epitope specific to USP48 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 119 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRecognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 3 DUSP domains.
Contains 1 ubiquitin-like domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- AAH67261 antibody
- Deubiquitinating enzyme 48 antibody
- DKFZp762M1713 antibody
All lanes : Anti-USP48 antibody (ab72226) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 119 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Detection of USP48 by Western Blot of Immunprecipitate.
ab72226 at 1µg/ml staining USP48 in HeLa whole cell lysate immunoprecipitated using ab72226 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.
ICC/IF image of ab72226 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Ghanem A et al. Catalytic domain of deubiquitinylase USP48 directs interaction with Rel homology domain of nuclear factor kappaB transcription factor RelA. Mol Biol Rep 46:1369-1375 (2019). Read more (PubMed: 30628021) »
- Zhou A et al. Gli1-induced deubiquitinase USP48 aids glioblastoma tumorigenesis by stabilizing Gli1. EMBO Rep 18:1318-1330 (2017). Read more (PubMed: 28623188) »