Key features and details
- Rabbit polyclonal to USP5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-USP5 antibody
See all USP5 primary antibodies
DescriptionRabbit polyclonal to USP5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Synthetic peptide within Human USP5 aa 75-125. The exact sequence is proprietary. (CAA62690.1).
Database link: P45974
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241311 was affinity purified using an epitope specific to USP5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 96 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionCleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger.
DomainThe UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
modificationsThe N-terminus is blocked.
- Information by UniProt
- Deubiquitinating enzyme 5 antibody
- Isopeptidase T antibody
- ISOT 1 antibody
All lanes : Anti-USP5 antibody (ab241311) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 96 kDa
Exposure time: 30 seconds
USP5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241311 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241311 at 1 µg/ml.
Lane 1: ab241311 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241311 has not yet been referenced specifically in any publications.