Overview

  • Product name
    Anti-USP5 antibody
    See all USP5 primary antibodies
  • Description
    Rabbit polyclonal to USP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 375 and 425 of Human USP5 (CAA62690.1).

  • Positive control
    • Human Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Stomach Adenocarcinoma and Testicular Seminoma.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Applications

Our Abpromise guarantee covers the use of ab84695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
    • Sequence similarities
      Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • Domain
      The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Information by UniProt
    • Database links
      • Alternative names
        • Deubiquitinating enzyme 5 antibody
        • Isopeptidase T antibody
        • ISOT 1 antibody
        • ISOT antibody
        • Ubiquitin carboxyl-terminal hydrolase 5 antibody
        • Ubiquitin isopeptidase T antibody
        • Ubiquitin specific peptidase 5 (isopeptidase T) antibody
        • Ubiquitin specific peptidase 5 antibody
        • Ubiquitin specific processing protease 5 antibody
        • Ubiquitin specific protease 5 (isopeptidase T) antibody
        • Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody
        • Ubiquitin thioesterase 5 antibody
        • Ubiquitin thiolesterase 5 antibody
        • Ubiquitin-specific-processing protease 5 antibody
        • UBP5_HUMAN antibody
        • Usp5 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)
        ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)
        ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using DAB staining.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)
        ab84695, at 1/500 dilution, staining USP5 in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry using DAB staining.

      References

      ab84695 has not yet been referenced specifically in any publications.

      Publishing research using ab84695? Please let us know so that we can cite the reference in this datasheet.

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