Anti-USP5 antibody (ab84695)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-USP5 antibody
See all USP5 primary antibodies -
Description
Rabbit polyclonal to USP5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residue 375 and 425 of Human USP5 (CAA62690.1).
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Positive control
- Human Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Skin Basal Cell Carcinoma, Stomach Adenocarcinoma and Testicular Seminoma.
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General notes
Concentration is optimized for IHC and not determined
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Application notes
IHC-P: 1/100 - 1/500.
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger. -
Domain
The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
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Database links
- Entrez Gene: 8078 Human
- Entrez Gene: 100174079 Orangutan
- Omim: 601447 Human
- SwissProt: P45974 Human
- SwissProt: Q5R407 Orangutan
- Unigene: 631661 Human
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Alternative names
- Deubiquitinating enzyme 5 antibody
- Isopeptidase T antibody
- ISOT 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP5 antibody (ab84695)ab84695, at 1/500 dilution, staining USP5 in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry using DAB staining.
Protocols
Datasheets and documents
References
ab84695 has not yet been referenced specifically in any publications.
