Recombinant Anti-USP5 antibody [EPR10453] (ab155993)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-USP5 antibody [EPR10453]
See all USP5 primary antibodies -
Description
Rabbit monoclonal [EPR10453] to USP5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human USP5 (Uniprot P45974).
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Positive control
- A549, K562, A375 and MCF7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10453 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 96 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger. -
Domain
The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
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Database links
- Entrez Gene: 8078 Human
- Entrez Gene: 22225 Mouse
- Entrez Gene: 297593 Rat
- Omim: 601447 Human
- SwissProt: P45974 Human
- SwissProt: P56399 Mouse
- Unigene: 631661 Human
- Unigene: 3571 Mouse
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Alternative names
- Deubiquitinating enzyme 5 antibody
- Isopeptidase T antibody
- ISOT 1 antibody
see all
Images
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![Western blot - Anti-USP5 antibody [EPR10453] (ab155993)](https://www.abcam.com/ps/products/155/ab155993/Images/ab155993-163318-anti-usp5-antibody-epr10453-western-blot.jpg)
Western blot - Anti-USP5 antibody [EPR10453] (ab155993)All lanes : Anti-USP5 antibody [EPR10453] (ab155993) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : A375 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 96 kDa
Datasheets and documents
References
ab155993 has not yet been referenced specifically in any publications.
