RecombinantRabMAb

Recombinant Anti-USP5 antibody [EPR10453] (ab155993)

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Western blot - Anti-USP5 antibody [EPR10453] (ab155993)
  • Anti-USP5 antibody [EPR10453] (ab155993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10453] to USP5
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-USP5 antibody [EPR10453]
    See all USP5 primary antibodies

  • Description

    Rabbit monoclonal [EPR10453] to USP5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to residues in Human USP5 (Uniprot P45974).

  • Positive control

    • A549, K562, A375 and MCF7 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • Target

    • Function

      Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

    • Sequence similarities

      Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.

    • Domain

      The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.

    • Post-translational
      modifications

      The N-terminus is blocked.
    • Information by UniProt

    • Database links

      • Alternative names

        • Deubiquitinating enzyme 5 antibody
        • Isopeptidase T antibody
        • ISOT 1 antibody
        • ISOT antibody
        • Ubiquitin carboxyl-terminal hydrolase 5 antibody
        • Ubiquitin isopeptidase T antibody
        • Ubiquitin specific peptidase 5 (isopeptidase T) antibody
        • Ubiquitin specific peptidase 5 antibody
        • Ubiquitin specific processing protease 5 antibody
        • Ubiquitin specific protease 5 (isopeptidase T) antibody
        • Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody
        • Ubiquitin thioesterase 5 antibody
        • Ubiquitin thiolesterase 5 antibody
        • Ubiquitin-specific-processing protease 5 antibody
        • UBP5_HUMAN antibody
        • Usp5 antibody
        see all

      Images

      • Western blot - Anti-USP5 antibody [EPR10453] (ab155993)
        Western blot - Anti-USP5 antibody [EPR10453] (ab155993)
        All lanes : Anti-USP5 antibody [EPR10453] (ab155993) at 1/1000 dilution

        Lane 1 : A549 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : A375 cell lysate
        Lane 4 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 96 kDa

      • Anti-USP5 antibody [EPR10453] (ab155993)
        Anti-USP5 antibody [EPR10453] (ab155993)

      References (1)

      Publishing research using ab155993? Please let us know so that we can cite the reference in this datasheet.

      ab155993 has been referenced in 1 publication.

      • Li J  et al. Deubiquitinase inhibitor degrasyn suppresses metastasis by targeting USP5-WT1-E-cadherin signalling pathway in pancreatic ductal adenocarcinoma. J Cell Mol Med 24:1370-1382 (2020). PubMed: 31845546

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