Recombinant
RabMAb

Anti-USP5 antibody [EPR10453] (ab155993)

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Overview

  • Product name
    Anti-USP5 antibody [EPR10453]
    See all USP5 primary antibodies
  • Description
    Rabbit monoclonal [EPR10453] to USP5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human USP5 (Uniprot P45974).

  • Positive control
    • A549, K562, A375 and MCF7 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 96 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • Target

    • Function
      Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
    • Sequence similarities
      Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • Domain
      The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Information by UniProt
    • Database links
      • Alternative names
        • Deubiquitinating enzyme 5 antibody
        • Isopeptidase T antibody
        • ISOT 1 antibody
        • ISOT antibody
        • Ubiquitin carboxyl-terminal hydrolase 5 antibody
        • Ubiquitin isopeptidase T antibody
        • Ubiquitin specific peptidase 5 (isopeptidase T) antibody
        • Ubiquitin specific peptidase 5 antibody
        • Ubiquitin specific processing protease 5 antibody
        • Ubiquitin specific protease 5 (isopeptidase T) antibody
        • Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody
        • Ubiquitin thioesterase 5 antibody
        • Ubiquitin thiolesterase 5 antibody
        • Ubiquitin-specific-processing protease 5 antibody
        • UBP5_HUMAN antibody
        • Usp5 antibody
        see all

      Images

      • Western blot - Anti-USP5 antibody [EPR10453] (ab155993)
        Western blot - Anti-USP5 antibody [EPR10453] (ab155993)
        All lanes : Anti-USP5 antibody [EPR10453] (ab155993) at 1/1000 dilution

        Lane 1 : A549 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : A375 cell lysate
        Lane 4 : MCF7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 96 kDa

      References

      ab155993 has not yet been referenced specifically in any publications.

      Publishing research using ab155993? Please let us know so that we can cite the reference in this datasheet.

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