Overview

  • Product name

    Anti-USP51 antibody
  • Description

    Rabbit polyclonal to USP51
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Pig, Gorilla
  • Immunogen

    Recombinant fragment: LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEP, corresponding to internal sequence amino acids 211-343 of Human USP51 (UniProt ID: Q70EK9).

  • Positive control

    • IHC-P: Human pancreas tissue WB: Human RT-4, U-251 MG, A-431, liver, and tonsil lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab121147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed in prostate, brain, lung, aorta and kidney.
  • Sequence similarities

    Belongs to the peptidase C19 family.
    Contains 1 UBP-type zinc finger.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deubiquitinating enzyme 51 antibody
    • Ubiquitin carboxyl-terminal hydrolase 51 antibody
    • Ubiquitin specific peptidase 51 antibody
    • Ubiquitin thioesterase 51 antibody
    • Ubiquitin thiolesterase 51 antibody
    • Ubiquitin-specific-processing protease 51 antibody
    • UBP51_HUMAN antibody
    • USP51 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab121147 at a 1/100 dilution. The image shows strong cytoplasmic positivity in exocrine glandular cells.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 21 2013

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