Anti-USP51 antibody (ab121147)
Key features and details
- Rabbit polyclonal to USP51
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-USP51 antibody -
Description
Rabbit polyclonal to USP51 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Pig, Gorilla -
Immunogen
Recombinant fragment: LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEP, corresponding to internal sequence amino acids 211-343 of Human USP51 (UniProt ID: Q70EK9).
-
Positive control
- IHC-P: Human pancreas tissue WB: Human RT-4, U-251 MG, A-431, liver, and tonsil lysates
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Tissue specificity
Expressed in prostate, brain, lung, aorta and kidney. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger. - Information by UniProt
-
Database links
- Entrez Gene: 158880 Human
- SwissProt: Q70EK9 Human
- Unigene: 40061 Human
-
Alternative names
- Deubiquitinating enzyme 51 antibody
- Ubiquitin carboxyl-terminal hydrolase 51 antibody
- Ubiquitin specific peptidase 51 antibody
see all
Images
-
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab121147 at a 1/100 dilution. The image shows strong cytoplasmic positivity in exocrine glandular cells.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab121147 has been referenced in 2 publications.
- Che X et al. FAT4-USP51 complex regulates the proliferation and invasion of endometrial cancer via Hippo pathway. Am J Transl Res 11:2784-2800 (2019). PubMed: 31217854
- Zhou Z et al. USP51 promotes deubiquitination and stabilization of ZEB1. Am J Cancer Res 7:2020-2031 (2017). PubMed: 29119051