Key features and details
- Rabbit polyclonal to USP6
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-USP6 antibody
DescriptionRabbit polyclonal to USP6
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human USP6 aa 1122-1359.
PLTPQGDELSKPRILAREVKKVDAQSSAGKEDMLLSKSPSSLSANISSSP KGSPSSSRKSGTSCPSSKNSSPNSSPRTLGRSKGRLRLPQIGSKNKPSSS KKNLDASKENGAGQICELADALSRGHMRGGSQPELVTPQDHEVALANGFL YEHEACGNGCGDGYSNGQLGNHSEEDSTDDQREDTHIKPIYNLYAISCHS GILSGGHYITYAKNPNCKWYCYNDSSCEELHPDEIDTD
Database link: P35125-1
- WB: Rat kidney tissue lysate. IHC: Human ovarian cancer tissue. ICC/IF: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000.|
FunctionDeubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
Tissue specificityTestis specific. Expressed in various cancer cell lines.
Involvement in diseaseNote=A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation.
Sequence similaritiesBelongs to the peptidase C19 family.
Contains 1 Rab-GAP TBC domain.
DomainThe Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6.
modificationsMonubiquitinated; ubiquitination is calmodulin and calcium dependent.
Cellular localizationCell membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
- Information by UniProt
- Deubiquitinating enzyme 6 antibody
- HRP1 antibody
- Proto-oncogene TRE-2 antibody
Anti-USP6 antibody (ab224725) at 1/500 dilution + rat kidney tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Observed band size: 159 kDa why is the actual band size different from the predicted?
Paraffin embedded human ovarian cancer tissue stained for USP6 with ab224725 (1/100 dilution) in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for USP6 (green) using ab224725 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224725 has not yet been referenced specifically in any publications.