• Product name
  • Description
    Goat polyclonal to USP6
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 773-786 of Human USP6.

  • Positive control
    • Human placenta
  • General notes
    GenBank Accession Number – NP_004496. Gene Ontology terms - lysosomal cysteine-type endopeptidase; nucleic acid binding; deubiquitination; oncogenesis; protein modification.



Our Abpromise guarantee covers the use of ab4553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).Can be blocked with Human USP6 peptide (ab23054). An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


  • Function
    Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
  • Tissue specificity
    Testis specific. Expressed in various cancer cell lines.
  • Involvement in disease
    Note=A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation.
  • Sequence similarities
    Belongs to the peptidase C19 family.
    Contains 1 Rab-GAP TBC domain.
  • Domain
    The Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6.
  • Post-translational
    Monubiquitinated; ubiquitination is calmodulin and calcium dependent.
  • Cellular localization
    Cell membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 6 antibody
    • HRP1 antibody
    • Proto-oncogene TRE-2 antibody
    • TRE17 antibody
    • TRE2 antibody
    • Ubiquitin carboxyl-terminal hydrolase 6 antibody
    • Ubiquitin specific protease 6 antibody
    • Ubiquitin thiolesterase 6 antibody
    • Ubiquitin-specific-processing protease 6 antibody
    • UBP6_HUMAN antibody
    • USP6 antibody
    see all


  • All lanes : Anti-USP6 antibody (ab4553) at 2 µg/ml

    Lane 1 : Human placenta lysate in RIPA buffer
    Lane 2 : Human placenta lysate in RIPA buffer with immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 90 kDa
    Observed band size: 90 kDa
    Additional bands at: 50 kDa (possible non-specific binding)


This product has been referenced in:
  • Urso ML  et al. Analysis of human skeletal muscle after 48 h immobilization reveals alterations in mRNA and protein for extracellular matrix components. J Appl Physiol 101:1136-48 (2006). Read more (PubMed: 16763108) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. This antibody is guaranted for use in WB for Human samples. The antibody was used at a concentration of 1-3 ug/ml, incubated overnight at 4 degrees. Blocking was done with 5% BSA. There is a non-specific band that appears a...

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Thank you for your enquiry. ab4553 is a fast track antibody. I have enclosed the fast track policy below. Essentially it is an antibody that we already sell at a reduced price since it is not fully characterized, therefore, I cannot send you a samp...

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