Overview

  • Product name

  • Description

    Rabbit polyclonal to Uteroglobin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ELISA, ICC/IF, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant rat uteroglobin His fusion protein.

  • General notes


    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Primary antibody notes

    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1600.
ELISA Use at an assay dependent concentration. PubMed: 21787397
ICC/IF Use at an assay dependent concentration. PubMed: 24367567
WB 1/5000. Predicted molecular weight: 10 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • Tissue specificity

    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • Sequence similarities

    Belongs to the secretoglobin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Blastokinin antibody
    • CC10 antibody
    • CC16 antibody
    • CCPBP antibody
    • CCSP antibody
    • Clara cell phospholipid binding protein antibody
    • Clara cell phospholipid-binding protein antibody
    • Clara cell specific 10 kD protein antibody
    • Clara cells 10 kDa secretory protein antibody
    • OTTHUMP00000236107 antibody
    • SCGB1A1 antibody
    • Secretoglobin family 1A member 1 antibody
    • Secretoglobin, family 1A, member 1 (uteroglobin) antibody
    • UG antibody
    • UGB antibody
    • UP-1 antibody
    • UP1 antibody
    • Urinary protein 1 antibody
    • Urine protein 1 antibody
    • UTER_HUMAN antibody
    • Uteroglobin antibody
    see all

Images

  • Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).

  • ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image. 

  • All lanes : Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution

    Lane 1 : Mouse lung whole tissue lysate with Milk
    Lane 2 : Mouse kidney whole tissue lysate with Milk

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 10 kDa


    Exposure time: 30 seconds

    See Abreview

  • ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

References

This product has been referenced in:

  • Hanson KM  et al. Apoptosis resistance in fibroblasts precedes progressive scarring in pulmonary fibrosis and is partially mediated by Toll-like receptor 4 activation. Toxicol Sci N/A:N/A (2019). Read more (PubMed: 31020321) »
  • Festa BP  et al. OCRL deficiency impairs endolysosomal function in a humanized mouse model for Lowe syndrome and Dent disease. Hum Mol Genet 28:1931-1946 (2019). Read more (PubMed: 30590522) »
See all 19 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9
Permeabilization
Yes - Triton X-100 0.1%
Specification
adult lung
Fixative
Formaldehyde

Mrs. Agnes Francois

Verified customer

Submitted Dec 11 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Feb 20 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung, bronchial epithelium)
Permeabilization
No
Specification
lung, bronchial epithelium
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2016

Application
Western blot
Sample
Mouse Cell lysate - other (urine)
Loading amount
5 µg
Specification
urine
Treatment
CLB (NP-40 based lysis buffer with protease inhibitors) , 10 min on ice
Gel Running Conditions
Reduced Denaturing (18)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 23 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Lung Parenchyma)
Specification
Human Lung Parenchyma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
1% BSA with 0.1% Triton-X in PBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 18°C

Abcam user community

Verified customer

Submitted Mar 15 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin (Ab970) 0.37%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Sep 09 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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