Anti-UTF1 antibody (ab105090)

Reviews (5)Q&A (2)

Overview

  • Product name

    Anti-UTF1 antibody
    See all UTF1 primary antibodies

  • Description

    Rabbit polyclonal to UTF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Pig, Macaque monkey

  • Immunogen

    Synthetic peptide corresponding to Human UTF1 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab119167)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: E14Tg2a; F9; IOUD2.

Properties

Applications

Our Abpromise guarantee covers the use of ab105090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • Function

    Acts as a transcriptional coactivator of ATF2.

  • Domain

    The leucine-zipper domain is required for coactivation activity. When this domain is deleted, the protein is able to stimulate transcription from a number of gene promoters.

  • Post-translational
    modifications

    Phosphorylated.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Form

      UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.

    • Alternative names

      • hUTF 1 antibody
      • hUTF1 antibody
      • Undifferentiated embryonic cell transcription factor 1 antibody
      • UTF 1 antibody
      • UTF1 antibody
      • UTF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-UTF1 antibody (ab105090)
      Western blot - Anti-UTF1 antibody (ab105090)
      All lanes : Anti-UTF1 antibody (ab105090) at 1 µg/ml

      Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
      Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
      Lane 3 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 36 kDa
      Observed band size: 46 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 36 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds


      The expression profile observed is consistent with what has been described in the literature (PMID:17785516).
    • Immunocytochemistry/ Immunofluorescence - Anti-UTF1 antibody (ab105090)
      Immunocytochemistry/ Immunofluorescence - Anti-UTF1 antibody (ab105090)
      ICC/IF image of ab105090 stained mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) mouse ES cells at 1µg/ml.

    References

    ab105090 has not yet been referenced specifically in any publications.

    Publishing research using ab105090? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-UTF1 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, postnatal day 8)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, postnatal day 8
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Mar 02 2019

    Western blot abreview for Anti-UTF1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Human Cell lysate - whole cell (undifferentiated human iPS cells)
    Specification
    undifferentiated human iPS cells
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 22 2014

    Question

    I would like to test ab105090 for use in IP.

    Read More
    Answer

    DISCOUNT CODE: ***
    Expiration date: January 11th. 2013

    I am very pleased to hear you would like to accept our offer and test ab105090 in IP. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-UTF1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Undifferentiated mouse ES cells)
    Specification
    Undifferentiated mouse ES cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - see blocking buffer
    Blocking step
    1% serum, 0.1% triton, 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-UTF1 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Undifferentiated human ES cells grown on feeders)
    Specification
    Undifferentiated human ES cells grown on feeders
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - see blocking buffer
    Blocking step
    1% serum, 0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1%
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 23 2011

    ChIP on chip abreview for Anti-UTF1 antibody

     Poor
    Abreviews
    abreview image
    Application
    ChIP on chip
    Sample
    Mouse Cell lysate - other (male germ cell culture)
    Specification
    male germ cell culture
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Microarray
    Positive control
    Sample for positive pulldown with this antibody was cultured mouse male germ cells with confirmed UTF1 expression by ICC. 30ug chromatin and 5ug antibody used per ChIP reaction.
    Negative control
    Input - gDNA from chromatin from same sample.
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 15 2011

    Question

    will test ab105090 in ChIP

    Read More
    Answer

    Thank you for your phone call. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab105090 in ChIP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

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