Anti-UTF1 antibody (ab105090)
Key features and details
- Rabbit polyclonal to UTF1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-UTF1 antibody
See all UTF1 primary antibodies -
Description
Rabbit polyclonal to UTF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cow, Human, Pig, Macaque monkey -
Immunogen
Synthetic peptide corresponding to Human UTF1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab119167) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: E14Tg2a; F9; IOUD2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Acts as a transcriptional coactivator of ATF2. -
Domain
The leucine-zipper domain is required for coactivation activity. When this domain is deleted, the protein is able to stimulate transcription from a number of gene promoters. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8433 Human
- Entrez Gene: 22286 Mouse
- Entrez Gene: 681901 Rat
- Omim: 604130 Human
- SwissProt: Q5T230 Human
- SwissProt: Q6J1H4 Mouse
- Unigene: 458406 Human
- Unigene: 10205 Mouse
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Form
UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP. -
Alternative names
- hUTF 1 antibody
- hUTF1 antibody
- Undifferentiated embryonic cell transcription factor 1 antibody
see all
Images
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All lanes : Anti-UTF1 antibody (ab105090) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The expression profile observed is consistent with what has been described in the literature (PMID:17785516). -
ICC/IF image of ab105090 stained mouse embryonic stem cells. The cells were fixed with 4% PFA (10min), permeabilized in 0.1% PBS-Triton X-100 for 5 min then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1hr. The cells were then incubated overnight at +4°C with ab105090 at 1/500 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). The secondary antibody (shown in green) was ab150081 Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab105090 has been referenced in 3 publications.
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Bi Y et al. Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation. Cell Rep 30:3917-3931.e5 (2020). PubMed: 32187559
- Wang M et al. Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis. Cell Stem Cell 23:599-614.e4 (2018). PubMed: 30174296