Overview

  • Product name

  • Description

    Rabbit polyclonal to Utrophin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to Human Utrophin aa 800-900 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107901)

  • Positive control

    • This antibody gave a positive signal in Human Skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab95443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 394 kDa (predicted molecular weight: 394 kDa).

Target

  • Function

    May play a role in anchoring the cytoskeleton to the plasma membrane.
  • Tissue specificity

    Muscle.
  • Sequence similarities

    Contains 2 CH (calponin-homology) domains.
    Contains 20 spectrin repeats.
    Contains 1 WW domain.
    Contains 1 ZZ-type zinc finger.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cytoplasm > cytoskeleton. Neuromuscular junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • DMDL antibody
    • DRP 1 antibody
    • DRP antibody
    • DRP-1 antibody
    • DRP1 antibody
    • Dystrophin like protein antibody
    • Dystrophin related protein 1 antibody
    • Dystrophin related protein antibody
    • Dystrophin-related protein 1 antibody
    • FLJ23678 antibody
    • OTTHUMP00000040139 antibody
    • UTRN antibody
    • UTRO_HUMAN antibody
    • Utrophin (homologous to dystrophin) antibody
    • Utrophin antibody
    see all

Images

  • Anti-Utrophin antibody (ab95443) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 394 kDa
    Observed band size: 394 kDa
    Additional bands at: 215 kDa, 31 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

References

ab95443 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293T)
Permeabilization
No
Specification
293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Miss. Bassma Khamaisi

Verified customer

Submitted Aug 02 2018

Answer

Thank you very much for contacting us. Yes, the immunogen has 83% homology with the mouse sequence, so I think it is likely to react. We of course cannot gaurantee this since we have not yet tested it in mouse.

However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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