Overview

  • Product name

    Anti-UTY antibody [19C9]
    See all UTY primary antibodies
  • Description

    Mouse monoclonal [19C9] to UTY
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control

    • Expressed UTY in 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab91236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/40. Predicted molecular weight: 150 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

References

This product has been referenced in:

  • Ahn J  et al. Target sequencing and CRISPR/Cas editing reveal simultaneous loss of UTX and UTY in urothelial bladder cancer. Oncotarget 7:63252-63260 (2016). Read more (PubMed: 27533081) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Bladder)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Treatment
lentivirus for gene knock-out
Specification
Bladder
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Jinwoo Ahn

Verified customer

Submitted May 20 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Skin fibroblasts of a male UTX-knockout mouse)
Permeabilization
Yes - 0.1% Triton-X
Specification
Skin fibroblasts of a male UTX-knockout mouse
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 24 2015

Answer

Vielen Dank für Ihre Anfrage.

Ich habe mir einmal das Immunogen dieses Antikörpers genauer angesehen, und obwohl die Homologie eigentlich unter 85% liegt bin ich sehr besorgt, dass dieser Antikörper mit dem UTX Protein kreuzreagiert. Ich habe mich mit unserer Datenblattabteilung in Verbindung gesetzt, da ich denke, dass die Kreuzreaktionen vermerkt werden sollte.

Desweiteren möchte ich Ihnen den ab91236 Anti-UTY antibody [19C9] als kostenlosen Ersatz zusenden, da dieser Antikörper nur UTY erkennt. Können Sie mir bitte mitteilen, wann Sie diesen Antikörper bestellt haben?



Ich hoffe, dies hilft Ihnen weiter. Ich freue mich auf Ihre Antwort.


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Question
Answer

Great to hear you are interested in the promotion. What is the catalog number you would like to test for reactivity to Utx? You do not need the discount code to purchase the product. The discount code is only required at the end of the process when you submit your Abreview, at which point you need to include your code in the 'Additional notes' section of the review. As a reminder, please speak with a Scientific Support staff member before purchase of any additional antibodies you wish to test in the future.  

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