Anti-UVRAG antibody (ab223345)
Key features and details
- Rabbit polyclonal to UVRAG
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-UVRAG antibody
See all UVRAG primary antibodies -
Description
Rabbit polyclonal to UVRAG -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human UVRAG (internal sequence).
Database link: Q9P2Y5 -
Positive control
- ICC/IF: SK-N-BE cells. WB: 293T and HeLa whole cell lysate (ab150035); Rat liver lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 78 kDa.
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Notes |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 78 kDa. |
Target
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Function
Versatile protein that is involved in regulation of differenent cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:24056303). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300).
Involved in maintaining chromosomal stability. Promotes DNA double-strand break (DSB) repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining (NHEJ) (PubMed:22542840). Required for centrosome stability and proper chromosome segregation (PubMed:22542840). -
Tissue specificity
Highly expressed in brain, lung, kidney and liver. -
Involvement in disease
A chromosomal aberration involving UVRAG has been observed in a patient with heterotaxy (left-right axis malformation). Inversion Inv(11)(q13.5;q25). -
Sequence similarities
Contains 1 C2 domain. -
Post-translational
modificationsPhosphorylated at Ser-498 by MTOR under basal conditions; increases the interaction with RUBCN implicated in inhibitory effect of RUBCN on PI3KC3 and decreases interaction with RAB7,A and VPS16 and VPS39 (indicative for a class C Vps complex, possibly the HOPS complex) (PubMed:25533187). -
Cellular localization
Late endosome. Lysosome. Early endosome. Endoplasmic reticulum. Midbody. Chromosome, centromere. Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network. Colocalization with early endosomes is only partial. - Information by UniProt
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Database links
- Entrez Gene: 7405 Human
- Entrez Gene: 308846 Rat
- Omim: 602493 Human
- SwissProt: Q9P2Y5 Human
- Unigene: 202470 Human
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Alternative names
- Beclin 1 binding protein antibody
- DHTX antibody
- Disrupted in heterotaxy antibody
see all
Images
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4% formaldehyde-fixed SK-N-BE cells stained for UVRAG (green) using ab223345 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223345 (D) Merge.
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All lanes : Anti-UVRAG antibody (ab223345) at 1/200 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 6 minutesBlocking: 5% skimmed milk for 1 hour at RT
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Anti-UVRAG antibody (ab223345) at 1/200 dilution + Rat liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 6 minutesBlocking: 5% skimmed milk for 1 hour at RT.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223345 has not yet been referenced specifically in any publications.