Overview

  • Product name

  • Description

    Rabbit polyclonal to UVRAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human UVRAG (internal sequence).
    Database link: Q9P2Y5

  • Positive control

    • ICC/IF: SK-N-BE cells. WB: 293T and HeLa whole cell lysate (ab150035); Rat liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 78 kDa.

Target

  • Function

    Versatile protein that is involved in regulation of differenent cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:24056303). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300).
    Involved in maintaining chromosomal stability. Promotes DNA double-strand break (DSB) repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining (NHEJ) (PubMed:22542840). Required for centrosome stability and proper chromosome segregation (PubMed:22542840).
  • Tissue specificity

    Highly expressed in brain, lung, kidney and liver.
  • Involvement in disease

    A chromosomal aberration involving UVRAG has been observed in a patient with heterotaxy (left-right axis malformation). Inversion Inv(11)(q13.5;q25).
  • Sequence similarities

    Contains 1 C2 domain.
  • Post-translational
    modifications

    Phosphorylated at Ser-498 by MTOR under basal conditions; increases the interaction with RUBCN implicated in inhibitory effect of RUBCN on PI3KC3 and decreases interaction with RAB7,A and VPS16 and VPS39 (indicative for a class C Vps complex, possibly the HOPS complex) (PubMed:25533187).
  • Cellular localization

    Late endosome. Lysosome. Early endosome. Endoplasmic reticulum. Midbody. Chromosome, centromere. Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network. Colocalization with early endosomes is only partial.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beclin 1 binding protein antibody
    • DHTX antibody
    • Disrupted in heterotaxy antibody
    • p63 antibody
    • UV radiation resistance associated antibody
    • UV radiation resistance associated gene antibody
    • UV radiation resistance associated gene protein antibody
    • UV radiation resistance-associated gene protein antibody
    • UVRAG antibody
    • UVRAG_HUMAN antibody
    • VPS38 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for UVRAG (green) using ab223345 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223345 (D) Merge.

  • All lanes : Anti-UVRAG antibody (ab223345) at 1/200 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG: HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 78 kDa


    Exposure time: 6 minutes


    Blocking: 5% skimmed milk for 1 hour at RT

  • Anti-UVRAG antibody (ab223345) at 1/200 dilution + Rat liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG: HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 78 kDa


    Exposure time: 6 minutes


    Blocking: 5% skimmed milk for 1 hour at RT.

References

ab223345 has not yet been referenced specifically in any publications.

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