Overview

  • Product name
    Anti-v-Myb + c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free
    See all v-Myb + c-Myb primary antibodies
  • Description
    Rabbit monoclonal [EP769Y] to v-Myb + c-Myb (phospho S11) - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, ChIP, Dot blotmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human c-Myb.

  • Positive control
    • IHC-P: human cervical carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab217945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 21296997
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance
      Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • C myb antibody
      • C myb protein antibody
      • Cmyb antibody
      • MYB antibody
      • Myb proto oncogene antibody
      • V myb myeloblastosis viral oncogene homolog antibody
      see all

    Images

    • Dot blot analysis of v-Myb + c-Myb (phospho S11/12) peptide (Lane 1) and v-Myb + c-Myb unmodified peptide (Lane 2) labeling v-Myb + c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).

    • Unpurified ab45150 staining human v-Myb / c-Myb in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).

    References

    This product has been referenced in:
    • Aisagbonhi OA  et al. Fine-Needle Aspiration of Epithelial-Myoepithelial Carcinoma of the Parotid Gland With Prominent Adenoid Cystic Carcinoma-Like Cribriform Features: Avoiding a Diagnostic Pitfall. Am J Clin Pathol N/A:N/A (2016). Read more (PubMed: 27614667) »
    • Li L  et al. Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer. Sci Signal 7:ra47 (2014). Read more (PubMed: 24847116) »
    See all 12 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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