Overview

  • Product name
    Anti-v-Myb + a-Myb antibody [5E11]
  • Description
    Mouse monoclonal [5E11] to v-Myb + a-Myb
  • Host species
    Mouse
  • Specificity
    ab10934 recognises v-myb and A-myb from Xenopus cells. The antibody does not recognise c-myb in human or mouse.
  • Tested applications
    Suitable for: IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Xenopus laevis
    Does not react with: Mouse, Human
  • Immunogen

    Recombinant v-myb protein, DNA binding protein.

  • Positive control
    • Xenopus testes lysate or tissue sections.
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab10934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
IP Use at an assay dependent concentration.

Target

  • Relevance
    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • myb antibody
      • Myb protein antibody

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Thank you for your email. We try our best in making datasheets very clear to our customers. We always publish entire information we have related to the products that includes Abreviews, publications and FAQs. This information is to help to get best results from our products. With scientific support we also work closely with our customers for solving their problems. In this case I apologize for the inconvenience caused due to misinterpretation of the published data. You being a value customer and in order to help you in your research I would like to recommend using ab45150 https://www.abcam.com/v-Myb-c-Myb-antibody-EP769Y-ab45150.html; this antibody was tested in human samples and is fully guaranteed to work. Let me know if you would like to accept my offer and then I will send a discount code for purchase of ab45150. I will look forward to hearing from you soon. Should you have any other question please do not hesitate to ask.

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    Question

    LOT NUMBER GR10374-2 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Human Cell lysate - whole cell (Breast cancer cell line) PRIMARY ANTIBODY Concentration or dilution: 1:250 <- 1:1000 Diluent buffer: PBS 1X Incubation time Over night: (16h) Incubation temperature: 4 oC. DETECTION METHOD ECL / ECLPlus / Odyssey POSITIVE AND NEGATIVE CONTROLS USED Datasheet do not mentioned any cell line for control. ANTIBODY STORAGE CONDITIONS When the antibodies come immediately aliquot and stored at -20 C, as recommended. SAMPLE PREPARATION Lysis buffer RIPA buffer Protease inhibitors: Yes. Complete mini (Roche) Phosphatase inhibitors: I don’t use. Reducing agent: B-mercaptoEthanol Boiling for ≥5 min? Yes. 5 min! AMOUNT OF PROTEIN LOADED Protein loaded ug/lane or cells/lane: 50 ug of protein per lane. ELECTROPHORESIS/GEL CONDITIONS 5) Percentage of gel: Reduced Denaturing (12%) TRANSFER AND BLOCKING CONDITIONS Type of membrane: Amersham Hybond ECL Nitrocellulose Membrane Protein transfer verified: Yes. Blocking agent and concentration: BSA 5% Blocking time: 1h Blocking temperature: Room temperature. SECONDARY ANTIBODY Secondary antibody: Non-Abcam antibody was used: Anti-mouse (Working with other antibodies in my lab) and conjugate with fluorophores Species:Goat Reacts against: Mouse Concentration or dilution 1:10000 (that is usual in my lab). Diluent buffer PBS 1X. Incubation time 1h HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 7 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Sonicate samples in one experiments and change dillutions of primary antibody. ADDITIONAL NOTES Dear colleagues In the last year I acquired 8 antibodies from Abcam. Some worked perfectly without adjustments to my methods. For others, I'm still making adjustments and at this time would abstain about them. However, this antibody [v-myb+ a-myb (MYBL1)] I could never detect any bands. I'm very disappointed with the quality of this antibody and request a replacement for it. Nevertheless, I would like to exchange the product for another one of your company. The antibody that would be most helpful at this point of my thesis would be the anti-CDC42 (ab64533). I hope that this partnership and preference that I gave to your company can help me in my application request. Thank you very much.

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    Answer

    Thank you for your enquiry regarding ab10934 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. The ab10934 was only tested using Xenopus lysates; the antibody does not react with human and mouse cells so I am sorry I do not understand why the antibody was used in human lysates although its non reactivity is clearly mentioned on the datasheet. Please check the datasheet; https://www.abcam.com/v-myb-a-myb-antibody-5e11-ab10934.html Using antibodies in non tested species or applications actually voids Abcam Abpromise guarantee. Please check the Abpromise Guarantee period at following link https://www.abcam.com/index.html?pageconfig=abpromise We have another antibody ab45150; https://www.abcam.com/v-Myb-c-Myb-antibody-EP769Y-ab45150.html which is tested in human and fully guaranteed; you may consider buying this. I have also had a look at the protocol and would like to ask few more details regarding the secondary antibody. It is mentioned that the secondary antibody is Fluorophore conjugated. Could you specify which fluorophore it was conjugated with? I presume it is conjugated with either HRP for ECL detection or con conjugated with Infrared dye for Odyssey. Could you also provide the order number of purchase? I will look forward to hearing from you soon.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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