Phospho anti-v-Myb + c-Myb (S12) antibody (ab78892)

Product name

Anti-v-Myb + c-Myb (phospho S12) antibody
See all v-Myb + c-Myb primary antibodies
Description

Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
Host species

Rabbit
Specificity

ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.
Tested applications

Suitable for: WB, ELISA, IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse
Immunogen

Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-S^P-D-E).
Positive control
- Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

Without Mg2+ and Ca2+
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab78892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/1000. Predicted molecular weight: 72 kDa.
ELISA		1/5000.
IHC-P		1/50 - 1/100.

Relevance

Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
Cellular localization

Nuclear
Database links
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Omim: 189990 Human
- SwissProt: P10242 Human
- SwissProt: P06876 Mouse
- Unigene: 654446 Human
Alternative names
- C myb antibody
- C myb protein antibody
- Cmyb antibody
- MYB antibody
- Myb proto oncogene antibody
- V myb myeloblastosis viral oncogene homolog antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution

Lane 1 : Extracts from HeLa cells treated
with Hu (2nM, 24hours)
Lane 2 : Extracts from HeLa cells treated
with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg

Lysates/proteins at 30 µg per lane.

Predicted band size: 72 kDa
Observed band size: 72 kDa

ab78892 has not yet been referenced specifically in any publications.

Publishing research using ab78892? Please let us know so that we can cite the reference in this datasheet.

Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)