Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
  • Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

Key features and details

  • Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-v-Myb + c-Myb (phospho S12) antibody

  • Description

    Rabbit polyclonal to v-Myb + c-Myb (phospho S12)

  • Host species

    Rabbit

  • Specificity

    ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).

  • Positive control

    • Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab78892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
IHC-P
1/50 - 1/100.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
IHC-P
1/50 - 1/100.

Target

  • Relevance

    Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • C myb antibody
      • C myb protein antibody
      • Cmyb antibody
      • MYB antibody
      • Myb proto oncogene antibody
      • V myb myeloblastosis viral oncogene homolog antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
    • Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution

      Lane 1 : Extracts from HeLa cells treated
      with Hu (2nM, 24hours)
      Lane 2 : Extracts from HeLa cells treated
      with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 72 kDa
      Observed band size: 72 kDa

    References (0)

    Publishing research using ab78892? Please let us know so that we can cite the reference in this datasheet.

    ab78892 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab78892.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com