Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

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Overview

  • Product name

    Anti-v-Myb + c-Myb (phospho S12) antibody

  • Description

    Rabbit polyclonal to v-Myb + c-Myb (phospho S12)

  • Host species

    Rabbit

  • Specificity

    ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.

  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).

  • Positive control

    • Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 72 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Relevance

    Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • C myb antibody
      • C myb protein antibody
      • Cmyb antibody
      • MYB antibody
      • Myb proto oncogene antibody
      • V myb myeloblastosis viral oncogene homolog antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
    • Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution

      Lane 1 : Extracts from HeLa cells treated
      with Hu (2nM, 24hours)
      Lane 2 : Extracts from HeLa cells treated
      with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 72 kDa
      Observed band size: 72 kDa

    References

    ab78892 has not yet been referenced specifically in any publications.

    Publishing research using ab78892? Please let us know so that we can cite the reference in this datasheet.

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