Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)

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Overview

  • Product name
    Anti-v-Myb + c-Myb (phospho S12) antibody
    See all v-Myb + c-Myb primary antibodies
  • Description
    Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
  • Host species
    Rabbit
  • Specificity
    ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).

  • Positive control
    • Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 72 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Relevance
    Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • C myb antibody
      • C myb protein antibody
      • Cmyb antibody
      • MYB antibody
      • Myb proto oncogene antibody
      • V myb myeloblastosis viral oncogene homolog antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
    • Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      Western blot - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
      All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution

      Lane 1 : Extracts from HeLa cells treated
      with Hu (2nM, 24hours)
      Lane 2 : Extracts from HeLa cells treated
      with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 72 kDa
      Observed band size: 72 kDa

    References

    ab78892 has not yet been referenced specifically in any publications.

    Publishing research using ab78892? Please let us know so that we can cite the reference in this datasheet.

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