Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)
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Overview
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Product nameAnti-v-Myb + c-Myb (phospho S12) antibody
See all v-Myb + c-Myb primary antibodies -
DescriptionRabbit polyclonal to v-Myb + c-Myb (phospho S12)
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Host speciesRabbit
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Specificityab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.
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Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).
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Positive control
- Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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PurityImmunogen affinity purified
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Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab78892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/1000. Predicted molecular weight: 72 kDa. | |
ELISA | 1/5000. | |
IHC-P | 1/50 - 1/100. |
Target
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RelevanceTranscriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Omim: 189990 Human
- SwissProt: P10242 Human
- SwissProt: P06876 Mouse
- Unigene: 654446 Human
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Alternative names
- C myb antibody
- C myb protein antibody
- Cmyb antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892)Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
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All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with Hu (2nM, 24hours)
Lane 2 : Extracts from HeLa cells treated
with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Datasheets and documents
References
ab78892 has not yet been referenced specifically in any publications.