Overview

  • Product name

  • Description

    Rabbit polyclonal to V2R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human V2R aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab133226)

  • Positive control

    • This antibody gave a positive signal in HEK293 whole cell lysate as well as the following tissue lysates: Human Fetal Kidney and Mouse Kidney.
  • General notes

     This product was previously labelled as AVPR V2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab109326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40,43 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificity

    Kidney.
  • Involvement in disease

    Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD) [MIM:300539]. This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis.
    Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI) [MIM:304800]; also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADHR antibody
    • Antidiuretic hormone receptor antibody
    • Arginine vasopressin receptor 2 antibody
    • AVP R2 antibody
    • AVPR 2 antibody
    • AVPR V2 antibody
    • AVPR2 antibody
    • DI1 antibody
    • DIR 3 antibody
    • DIR antibody
    • DIR3 antibody
    • MGC126533 antibody
    • MGC138386 antibody
    • NDI antibody
    • Nephrogenic diabetes insipidus antibody
    • Renal type arginine vasopressin receptor antibody
    • Renal-type arginine vasopressin receptor antibody
    • V2 receptor antibody
    • V2R antibody
    • V2R_HUMAN antibody
    • Vasopressin V2 antibody
    • Vasopressin V2 receptor antibody
    see all

Images

  • All lanes : Anti-V2R antibody (ab109326) at 1 µg/ml (Milk blocking - 5%)

    Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 26 kDa, 72 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • Anti-V2R antibody (ab109326) at 1 µg/ml (Milk blocking - 3%) + Kidney (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 12 minutes


    V2R contains a potential glycosylation site (SwissProt) which may explain the banding pattern observed.

References

This product has been referenced in:

  • Wang F  et al. Site-1 protease-derived soluble (pro)renin receptor targets vasopressin receptor 2 to enhance urine concentrating capability. JCI Insight 4:N/A (2019). Read more (PubMed: 30944256) »
  • Czarzasta K  et al. Effect of Chronic Kidney Disease on Changes in Vasopressin System Expression in the Kidney Cortex in Rats with Nephrectomy. Biomed Res Int 2018:2607928 (2018). Read more (PubMed: 30013980) »
See all 3 Publications for this product

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