Key features and details
- Chicken polyclonal to V5 tag
- Suitable for: WB
- Isotype: IgY
Product nameAnti-V5 tag antibody
See all V5 tag primary antibodies
DescriptionChicken polyclonal to V5 tag
Tested applicationsSuitable for: WBmore details
Synthetic peptide corresponding to Human V5 tag conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Drosophila S2 cells transfected with V5 epitope tagged protein. No band was observed in the negative control.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab107244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Block using 3% milk.|
RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
All lanes : Anti-V5 tag antibody (ab107244) at 1 µg/ml
Lane 1 : Drosophila S2 cells transfected with V5 epitope tagged MSI-A
Lane 2 : Drosophila S2 Negative Control
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
ab107244 has not yet been referenced specifically in any publications.