The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Block using 3% milk.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
GKPIPNPLLGLDST epitope tag antibody
GKPIPNPLLGLDST tag antibody
Protein Rev antibody
Regulator of expression of viral proteins antibody
V5 epitope tag antibody
Western blot - Anti-V5 tag antibody (ab107244)
All lanes : Anti-V5 tag antibody (ab107244) at 1 µg/ml
Lane 1 : Drosophila S2 cells transfected with V5 epitope tagged MSI-A Lane 2 : Drosophila S2 Negative Control
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution