Overview

  • Product name
  • Description
    Chicken polyclonal to V5 tag
  • Host species
    Chicken
  • Specificity
    Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:100,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP conjugated goat anti chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. Antibody concentration was determined by extinction coefficient : O.D. 1.32 at 280nM equals 1.0 mg of IgY (IgG).
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab9113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/5000. Can be blocked with V5 tag peptide (ab15829).
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • ab9113 staining human fibrosarcoma cells (HT1080) by ICC/IF. Cells were transfected with V5- tagged transmembrane protein and PFA fixed without undergoing permeabilization and blocking steps. The primary antibody was used at 10µg/ml and incubated with the sample for 1 hour at 20°C. An Alexa Fluor® 546 conjugated donkey polyclonal anti-chicken (H+L) antibody, diluted 1/500, was used as the secondary.

    See Abreview

References

This product has been referenced in:
See all 6 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibrosarcoma)
Specification
fibrosarcoma
Fixative
Paraformaldehyde
Permeabilization
No

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Submitted Dec 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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