Anti-V5 tag antibody (ab9116)
Key features and details
- Rabbit polyclonal to V5 tag
- Suitable for: WB, ELISA, IP, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Rabbit polyclonal to V5 tag -
Host species
Rabbit -
Specificity
ELISA: V5 tag antibody - ChIP Grade (ab9116) specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
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Tested applications
Suitable for: WB, ELISA, IP, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg . -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (7) |
Use a concentration of 0.05 - 0.5 µg/ml. Can be blocked with V5 tag peptide (ab15829).
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| ELISA |
Use at an assay dependent concentration.
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| IP | (2) |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 0.5 - 5 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.05 - 0.5 µg/ml. Can be blocked with V5 tag peptide (ab15829). |
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ELISA
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 0.5 - 5 µg/ml. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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Western blot - Anti-V5 tag antibody (ab9116)This image is courtesy of an anonymous AbreviewAll lanes : Anti-V5 tag antibody (ab9116) at 1/5000 dilution
Lanes 1-3 : Cells transfected with v5-tagged protein.
Lanes 4-5 : Untransfected cells.
Secondary
Lanes 1-3 : Anti-rabbit Ig-HRP at 1/20000 dilution
Lanes 4-5 : Anti-rabbit Ig-HRP at 1/20000 dilution
Mouse ES cells lysate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (115)
ab9116 has been referenced in 115 publications.
- Koppenhafer SL et al. Inhibition of the ATR-CHK1 Pathway in Ewing Sarcoma Cells Causes DNA Damage and Apoptosis via the CDK2-Mediated Degradation of RRM2. Mol Cancer Res 18:91-104 (2020). PubMed: 31649026
- Deng M et al. Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents. Sci Adv 6:N/A (2020). PubMed: 32917705
- Gibbs ZA et al. The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFß signaling in triple-negative breast cancer. Elife 9:N/A (2020). PubMed: 32515734
- Edwards DS et al. BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation. Cell Rep 32:108166 (2020). PubMed: 32966794
- Ball DP et al. Caspase-1 interdomain linker cleavage is required for pyroptosis. Life Sci Alliance 3:N/A (2020). PubMed: 32051255
Customer reviews and Q&As
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