Anti-V5 tag antibody (ab9116)
- Datasheet
- References (86)
- Protocols
Overview
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Product name
Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Rabbit polyclonal to V5 tag -
Host species
Rabbit -
Specificity
ELISA: V5 tag antibody - ChIP Grade (ab9116) specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
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Tested applications
Suitable for: ICC/IF, ChIP, WB, ELISA, IP, ChIP/Chip, IHC-P, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
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Positive control
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) can be used as a positive control in WB. Ab5395
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg . -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 2 - 10 µg/ml. PubMed: 18575576 | |
| ChIP | Use at an assay dependent concentration. PubMed: 17289569 | |
| WB | Use a concentration of 0.05 - 0.5 µg/ml. Can be blocked with V5 tag peptide (ab15829). | |
| ELISA | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| ChIP/Chip | Use at an assay dependent concentration. PubMed: 17289569 | |
| IHC-P | Use a concentration of 0.5 - 5 µg/ml. | |
| ICC | Use at an assay dependent concentration. PubMed: 17012257 |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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Western blot - Anti-V5 tag antibody - ChIP Grade (ab9116)This image is courtesy of an anonymous AbreviewAll lanes : Anti-V5 tag antibody (ab9116) at 1/5000 dilution
Lanes 1-3 : Cells transfected with v5-tagged protein.
Lanes 4-5 : Untransfected cells.
Secondary
Lanes 1-3 : Anti-rabbit Ig-HRP at 1/20000 dilution
Lanes 4-5 : Anti-rabbit Ig-HRP at 1/20000 dilution
Mouse ES cells lysate. -
Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody - ChIP Grade (ab9116)This image is courtesy of an anonymous Abreviewab9116 staining V5 tag in the Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 3% serum for 1 hour at 4°C. Samples were incubated with primary antibody (1/300) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/300).
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ChIP - Anti-V5 tag antibody (ab9116)Image from Roca H et al., PLoS One. 2013; 8(): e76773 (Figure S2, F) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ChIP qPCR: The graph on the left represents the input chromatin of PC3-EMT14-OVOL2 relative to empty vector (EV) control. The graph on the right depicts the ChIP DNA using V5 antibody (ab9116). The V5 epitope was included at the C-terminus of the expressed OVOL2. Results were normalized to input controls and graphs are relative to EV. Graphs show mean +/- sem.
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Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody (ab9116)Image from Timinszky G. et.al., PLoS ONE. 2008; 3(6): e2506 (Fig. 2) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab9116 at 1/1000 dilution staining V5 in V5-Pgc transfected Drosophila Kc cells by Immunocytochemistry/ Immunofluorescence. Kc cells were fixed in 4% formaldehyde for 10 minutes, washed in PBS-Tween 20 (0.05%) and blocked in 5% milk in PBS-Tween 20 for 1 hour. Cells were incubated with primary antibody at 4°C overnight and the secondary antibody at room temperature for 3 hours.
Protocols
References
This product has been referenced in:
- Parameswaran S et al. Molecular characterization of an MLL1 fusion and its role in chromosomal instability. Mol Oncol 13:422-440 (2019). Read more (PubMed: 30548174) »
- Borzooee F & Larijani M Pichia pastoris as a host for production and isolation of mutagenic AID/APOBEC enzymes involved in cancer and immunity. N Biotechnol 51:67-79 (2019). Read more (PubMed: 30822538) »
