Anti-V5 tag antibody (ab9116)
Key features and details
- Rabbit polyclonal to V5 tag
- Suitable for: WB, ELISA, IP, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Rabbit polyclonal to V5 tag -
Host species
Rabbit -
Specificity
ELISA: V5 tag antibody - ChIP Grade (ab9116) specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
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Tested applications
Suitable for: WB, ELISA, IP, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg . -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (7) |
Use a concentration of 0.05 - 0.5 µg/ml. Can be blocked with V5 tag peptide (ab15829).
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ELISA |
Use at an assay dependent concentration.
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IP | (2) |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 0.5 - 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.05 - 0.5 µg/ml. Can be blocked with V5 tag peptide (ab15829). |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 0.5 - 5 µg/ml. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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All lanes : Anti-V5 tag antibody (ab9116) at 1/5000 dilution
Lanes 1-3 : Cells transfected with v5-tagged protein.
Lanes 4-5 : Untransfected cells.
Secondary
Lanes 1-3 : Anti-rabbit Ig-HRP at 1/20000 dilution
Lanes 4-5 : Anti-rabbit Ig-HRP at 1/20000 dilution
Mouse ES cells lysate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (152)
ab9116 has been referenced in 152 publications.
- Nicosia L et al. Pharmacological inhibition of LSD1 triggers myeloid differentiation by targeting GSE1 oncogenic functions in AML. Oncogene 41:878-894 (2022). PubMed: 34862459
- Páez-Moscoso DJ et al. A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis. Nat Commun 13:1067 (2022). PubMed: 35217638
- Kalyan G et al. Protein interactions with metallothionein-3 promote vectorial active transport in human proximal tubular cells. PLoS One 17:e0267599 (2022). PubMed: 35503771
- Song PX et al. Septin 9 and phosphoinositides regulate lysosome localization and their association with lipid droplets. iScience 25:104288 (2022). PubMed: 35573204
- Gao Y et al. Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83. Front Physiol 13:872889 (2022). PubMed: 35574494