Overview

  • Product name
  • Description
    Goat polyclonal to V5 tag
  • Host species
    Goat
  • Specificity
    Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, ICC, IHC - Wholemountmore details
  • Immunogen

    Synthetic peptide:

    GKPIPNPLLGLDST

    (V5) conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/30000. Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent concentration.

Use at a dilution of: Coating: 1:100 - 1:500 Primary: 1:1000 - 1:30,000.

ICC/IF Use at an assay dependent concentration.
ICC 1/200 - 1/2000.
IHC - Wholemount 1/400.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • All lanes : Anti-V5 tag antibody (ab9137) at 0.04 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg

References

This product has been referenced in:
See all 6 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.01% Triton-x 100
Specification
Brain
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 4°C
Fixative
Paraformaldehyde

Terry Yin

Verified customer

Submitted Aug 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T cells transfected with a solluble V5-tagge)
Specification
HEK293T cells transfected with a solluble V5-tagge
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% TritonX-100 in PBS for 15 minutes
Blocking step
Image-iT® FX Signal Enhancer (LifeTechnologies) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Mr. David Sanchez Martin

Verified customer

Submitted Jan 11 2013

Answer

I am very pleased to hear you would like to accept our offer and test ab9137 in ICC/IF and ab81397 in ICC. Each code will give you the VALUE of the product off an order before the expiration date. To redeem this offer, please submit an Abreview for ab9137 in ICC/IF and ab81397 in ICC. Each code is specific to theassociated antibody and is inactive until included in your submitted Abreview so be sure to add them to the Additional Comments section. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/AbTrial

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (Hindbrain)
Specification
Hindbrain

Ms. Sana Zakaria

Verified customer

Submitted Oct 02 2012

Answer

Thank you for your reply.
I am pleased to hear you would like to participate in the testing discount scheme. The scheme is as you say, regardless of whether the result is a negative or a positive, you would still be entitled to free primary antibody of your choice (or the equivalent price of ab9137 off any product in our catalogue).
As requested, I have generated the discount code for your as follows:
xxxxxxx
Please quote this code in the "Additional comments" section when submitting your Abreview. Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 20th of November 2012.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

Thank you for contacting us.
Unfortunately, as you have discovered from the datasheet of the anti-V5 antibody (ab9137), this antibody has not been used in whole mount IHC to our knowledge as yet. This does not mean that the antibody would not work in this application, simply that we have not tested it and we would therefore not be able to guarantee this. We are not able to provide sample sizes for any of our products. This is due to the size of our catalogue and the logistics this would involve. However, if you would like to purchase ab9137 and use it with your wholemount IHC staining, we can offer you a testing discount. This offer means that if you purchase ab9137 as normal, test the antibody with your samples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab9137 off any product).
More information on this offer can be found here:
www.abcam.com/collaborationdiscount
Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.
If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab9137.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Answer

The V5 epitope tag is used in transfection procedures in which the gene of interest is cloned in frame with the codons for the V5 epitope (GKPIPNPLLGLDST). The resulting expressed protein is tagged with the V5 epitope, which can be tracked with antibodies to the tag. KLH was used as a carrier in order to elicit an immune response to the synthetic V5 peptide, which is too small to generate an antibody response without conjugation to a larger carrier protein. Best of luck with your immunoelecton microspopy. I am not aware of the use of our V5 antibodies in EM, and would certainly appreciate hearing of your results.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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