Product nameAnti-V5 tag antibody
See all V5 tag primary antibodies
DescriptionGoat polyclonal to V5 tag
SpecificityAntibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).
Tested applicationsSuitable for: WB, ELISA, ICC/IF, ICC, IHC - Wholemountmore details
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
Light chain typeunknown
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab9137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/30000. Can be blocked with V5 tag peptide (ab15829).|
|ELISA||Use at an assay dependent concentration.
Use at a dilution of: Coating: 1:100 - 1:500 Primary: 1:1000 - 1:30,000.
|ICC/IF||Use at an assay dependent concentration.|
|ICC||1/200 - 1/2000.|
|IHC - Wholemount||1/400.|
RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
This product has been referenced in:
- Fossat N et al. C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47. EMBO Rep : (2014). Read more (PubMed: 24916387) »
- Wiradjaja F et al. Regulation of PDGFC signalling and extracellular matrix composition by FREM1 in mice. Dis Model Mech 6:1426-33 (2013). Read more (PubMed: 24046351) »