Anti-V5 tag antibody (ab9137)

Goat polyclonal V5 tag antibody. Validated in WB, ELISA, ICC, IHC, ICC/IF. Cited in 6 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name
  • Description
    Goat polyclonal to V5 tag
  • Host species
    Goat
  • Specificity
    Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, ICC, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide:

    GKPIPNPLLGLDST

    (V5) conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/30000. Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent concentration.

Use at a dilution of: Coating: 1:100 - 1:500 Primary: 1:1000 - 1:30,000.

ICC/IF Use at an assay dependent concentration.
ICC 1/200 - 1/2000.
IHC - Wholemount 1/400.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • All lanes : Anti-V5 tag antibody (ab9137) at 0.04 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg

References

This product has been referenced in:
See all 6 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.01% Triton-x 100
Specification
Brain
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 4°C
Fixative
Paraformaldehyde

Terry Yin

Verified customer

Submitted Aug 03 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T cells transfected with a solluble V5-tagge)
Specification
HEK293T cells transfected with a solluble V5-tagge
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% TritonX-100 in PBS for 15 minutes
Blocking step
Image-iT® FX Signal Enhancer (LifeTechnologies) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Mr. David Sanchez Martin

Verified customer

Submitted Jan 11 2013

Application
IHC - Wholemount
Sample
Mouse Tissue (Hindbrain)
Specification
Hindbrain

Ms. Sana Zakaria

Verified customer

Submitted Oct 02 2012

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