Anti-V5 tag antibody (ab95038)

Submit a review Q&A (3)References (4)

Overview

  • Product name
    Anti-V5 tag antibody
    See all V5 tag primary antibodies
  • Description
    Goat polyclonal to V5 tag
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, WB, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    V5 tag (GKPIPNPLLGLDST) coupled to KLH

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.03 - 10 µg/ml. Coating: 2 - 10 ug/ml; Primary: 0.03 - 1 ug/ml
WB Use a concentration of 0.03 - 1 µg/ml. Colorimetric: 0.1 - 1 ug/ml; Chemiluminescent: 0.03 - 1 ug/ml
ICC Use a concentration of 0.5 - 5 µg/ml.
IHC-P Use at an assay dependent dilution.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • Western blot - Anti-V5 tag antibody (ab95038)
    Western blot - Anti-V5 tag antibody (ab95038)
    A, cells expressing 315-HARE, 190-HARE, or EV alone were cultured in T-25 flasks until confluent, washed, scraped, and lysed. Cell lysates (100ug of protein) were immunoprecipitated (IP) with anti-Tyr(P) mAb and the immune complexes were subjected to 5% SDS-PAGE and Western blot (WB) analysis using anti-V5 pAb. Cells expressing 315-HARE (B) or 190-HARE (C) cDNA were grown until confluent, washed, incubated for 1 h in medium without serum, and then incubated with (HA) or without (Ctr) 5ug/ml HA for 20 min. The cells were lysed and 200 ug of total lysate protein was immunoprecipitated with anti-HARE mAb-30. Immune complexes were collected and subjected to 5% SDS-PAGE and Western analysis using anti- Tyr(P) mAb. The same blots in B and C were reprobed with anti-V5 Ab (D and E, respectively). Immunopurification using non-immune mouse IgG served as another control in B–E. The solid and open arrows indicate the positions of the 315-HARE and 190-HARE, respectively.
  • Western blot - Anti-V5 tag antibody (ab95038)
    Western blot - Anti-V5 tag antibody (ab95038)
    All lanes : Anti-V5 tag antibody (ab95038) at 0.4 µg/ml

    Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
    Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
    Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

    Developed using the ECL technique.

    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V5 tag antibody (ab95038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V5 tag antibody (ab95038)Image from Faraji F et al., PLoS Genet 8(9): e1002926.. Fig 4.; doi: 10.1371/journal.pgen.1002926. Epub 2012 Sep 20.
    Immunohistochemical analysis of murine mammary tumour tissue, expressing vector (left) or V5-tagged Cadm1 (right), staining V5 tag with ab95038.

References

This product has been referenced in:
  • Akhtar AA  et al. A transposon-mediated system for flexible control of transgene expression in stem and progenitor-derived lineages. Stem Cell Reports 4:323-31 (2015). WB, IHC ; Mouse . Read more (PubMed: 25702640) »
  • Breunig JJ  et al. Ets Factors Regulate Neural Stem Cell Depletion and Gliogenesis in Ras Pathway Glioma. Cell Rep 12:258-271 (2015). Mouse . Read more (PubMed: 26146073) »
See all 4 Publications for this product

Publishing research using ab95038? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Hello
Thank you very much for your email. Yes, I would like to submit ab95038
IHC-P images in exchange for a discount, please issue the discount code .
Thank you again.
Regards,

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Answer

Thank you for your reply. I am very pleased to hear you would like to use ab95038 in IHC-P and send us an image of your results.

DISCOUNT CODE: ########
Expiration date: ######


This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with the image for IHC-P and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

If you have any further questions, please do not hesitate to contact me. We look forward to receiving your Abreview and wish you luck with your research.

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Question

Hi

Thank you very much for this. There is one little question regarding these
images- is the Tyr antibody used for immunoprecipitation in figure A an
anti-pan-Tyrosine phosphate antibody or is it specific exclusively for the
HARE proteins? If only two proteins are selectively enriched in lanes 2 and
3 in fiure A, then what looks as a very clean blot could be due to lack of
other protein rather than the anti-V5 antibody being very specific. I would
be very grateful if you could find this out.

I will provide IHC-P images for your records (can also provide WB images
from whole cell lysates and tumour homogenates as I will run these as
controls with your antibody).

please also let me know the most convenient way of ordering your antibodies.
Thank you very much.
Regards,

Read More
Answer

Thank you for your reply.

I am very sorry to let you know that as the western blot images provided are from external testing, we have no further information to provide on this occasion. My apologies for the inconvenience in this case.

As previously discussed, we do not currently have an image of ab95038 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab95038 now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody. It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab95038 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab95038 so please wait for my reply before ordering.

2. Purchase ab95038 either by:
phone:01223 696000
fax: 01223 771600
online from the datasheet: https://www.abcam.com
or by email to our customer service department: mailto:orders@abacam,com

3. Use it in IHC-P.

4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

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Question

Do you have any WB or IHC images for ab95038

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Answer

Thank you for your recent telephone enquiry.

I am pleased to attach some WB images from ab95038. I hope these will be helpful to you. I am sorry there are currently no IHC-P images available. Please let me know before purchasing if you would like to provide an Abreview with IHC-P images, as you may be eligable for our image discount.

If you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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