Anti-V5 tag antibody (ab95038)
Key features and details
- Goat polyclonal to V5 tag
- Suitable for: ELISA, WB, ICC, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Goat polyclonal to V5 tag -
Host species
Goat -
Tested applications
Suitable for: ELISA, WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
V5 tag (GKPIPNPLLGLDST) coupled to KLH
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 0.03 - 10 µg/ml. Coating: 2 - 10 ug/ml; Primary: 0.03 - 1 ug/ml
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| WB |
Use a concentration of 0.03 - 1 µg/ml. Colorimetric: 0.1 - 1 ug/ml; Chemiluminescent: 0.03 - 1 ug/ml
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| ICC |
Use a concentration of 0.5 - 5 µg/ml.
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| IHC-P |
Use at an assay dependent dilution.
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| Notes |
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ELISA
Use a concentration of 0.03 - 10 µg/ml. Coating: 2 - 10 ug/ml; Primary: 0.03 - 1 ug/ml |
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WB
Use a concentration of 0.03 - 1 µg/ml. Colorimetric: 0.1 - 1 ug/ml; Chemiluminescent: 0.03 - 1 ug/ml |
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ICC
Use a concentration of 0.5 - 5 µg/ml. |
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IHC-P
Use at an assay dependent dilution. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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Western blot - Anti-V5 tag antibody (ab95038)A, cells expressing 315-HARE, 190-HARE, or EV alone were cultured in T-25 flasks until confluent, washed, scraped, and lysed. Cell lysates (100ug of protein) were immunoprecipitated (IP) with anti-Tyr(P) mAb and the immune complexes were subjected to 5% SDS-PAGE and Western blot (WB) analysis using anti-V5 pAb. Cells expressing 315-HARE (B) or 190-HARE (C) cDNA were grown until confluent, washed, incubated for 1 h in medium without serum, and then incubated with (HA) or without (Ctr) 5ug/ml HA for 20 min. The cells were lysed and 200 ug of total lysate protein was immunoprecipitated with anti-HARE mAb-30. Immune complexes were collected and subjected to 5% SDS-PAGE and Western analysis using anti- Tyr(P) mAb. The same blots in B and C were reprobed with anti-V5 Ab (D and E, respectively). Immunopurification using non-immune mouse IgG served as another control in B–E. The solid and open arrows indicate the positions of the 315-HARE and 190-HARE, respectively.
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Western blot - Anti-V5 tag antibody (ab95038)All lanes : Anti-V5 tag antibody (ab95038) at 0.4 µg/ml
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Developed using the ECL technique.
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V5 tag antibody (ab95038)Image from Faraji F et al., PLoS Genet 8(9): e1002926.. Fig 4.; doi: 10.1371/journal.pgen.1002926. Epub 2012 Sep 20.Immunohistochemical analysis of murine mammary tumour tissue, expressing vector (left) or V5-tagged Cadm1 (right), staining V5 tag with ab95038.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab95038 has been referenced in 6 publications.
- Soliman SHA et al. Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. Sci Rep 10:7138 (2020). PubMed: 32346083
- Kaczmarczyk L et al. Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice. PLoS Biol 17:e3000374 (2019). PubMed: 31393866
- Akhtar AA et al. A transposon-mediated system for flexible control of transgene expression in stem and progenitor-derived lineages. Stem Cell Reports 4:323-31 (2015). WB, IHC ; Mouse . PubMed: 25702640
- Breunig JJ et al. Ets Factors Regulate Neural Stem Cell Depletion and Gliogenesis in Ras Pathway Glioma. Cell Rep 12:258-271 (2015). Mouse . PubMed: 26146073
- Strobel B et al. Riboswitch-mediated Attenuation of Transgene Cytotoxicity Increases Adeno-associated Virus Vector Yields in HEK-293 Cells. Mol Ther 23:1582-91 (2015). PubMed: 26137851
- Faraji F et al. Cadm1 is a metastasis susceptibility gene that suppresses metastasis by modifying tumor interaction with the cell-mediated immunity. PLoS Genet 8:e1002926 (2012). IHC-P . PubMed: 23028344