Overview

  • Product name
    Anti-V5 tag antibody (Agarose)
    See all V5 tag primary antibodies
  • Description
    Goat polyclonal to V5 tag (Agarose)
  • Host species
    Goat
  • Conjugation
    Agarose
  • Tested applications
    Suitable for: IPmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Full length native

    GKPIPNPLLGLDST

    (V5) (purified): conjugated to KLH.

  • General notes
    Provided as 400µl of slurry (200µl gel + 200µl buffer). 100µg of the affinity purified antibody is attached to the gel at a ratio of 500µg antibody/ml of gel or 250µg IgG/ml of 50% slurry.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Properties

Applications

Our Abpromise guarantee covers the use of ab1229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

References

This product has been referenced in:
  • Liu T  et al. Glycosylation controls sodium-calcium exchanger 3 sub-cellular localization during cell cycle. Eur J Cell Biol 97:190-203 (2018). Read more (PubMed: 29526322) »
  • Yalla K  et al. FBXW7 regulates DISC1 stability via the ubiquitin-proteosome system. Mol Psychiatry 23:1278-1286 (2018). Read more (PubMed: 28727686) »
See all 13 Publications for this product

Customer reviews and Q&As

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Application
Immunoprecipitation
Immuno-precipitation step
Other - ab1229
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Treatment
transfected with plasmid DNA
Total protein in input
5e+006 cells

Ms. Christina Engel

Verified customer

Submitted Oct 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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